Zarandia Maia, Tsertsvadze Tengiz, Carr Jean K, Nadai Yuka, Sanchez Jose L, Nelson And Kenrad
Georgian Infectious Diseases, AIDS and Clinical Immunology Research Center, Tbilisi 38600, Georgia.
AIDS Res Hum Retroviruses. 2006 May;22(5):470-6. doi: 10.1089/aid.2006.22.470.
The genetic diversity and genotypic drug susceptibility of HIV-1 strains circulating in the Republic of Georgia, formerly part of the Soviet Union, were investigated for first time. Forty-eight HIV-positive drug-naive Georgian individuals contributed PBMC DNA between 1998 and 2003. On the basis of phylogenetic analyses of partial pol sequences, the predominant HIV-1 genetic forms were subtype A (70%), followed by subtype B (26%); both genetic forms were carried by injecting drug users and heterosexuals. There was also one subtype C (2%) and one CRF18_cpx (2%). The Georgian subtype A strains clustered with subtype A from Russia, designated A(FSU). Twelve of the subtype A strains (25%) contained the secondary protease inhibitor mutation V77I and 9 also had two other silent mutations. This "V77I haplotype" marks one particular genetic lineage of the epidemic in the former Soviet Union. Two strains (4%) carried antiretroviral (ARV) drug resistance mutations. Nearly full-length genome sequences of five Georgian strains were also completed. Two, 98GEMZ011 (subtype A) and 98GEMZ003 (subtype B), closely resembled the parental strains that recombined to create CRF03_AB. The use of these parental strains in the analysis revealed an additional segment of subtype A in CRF03_AB. Thus, the HIV-1 epidemic in Georgia was composed of a mixture of subtype A(FSU) and subtype B.
对格鲁吉亚共和国（前苏联的一部分）流行的HIV-1毒株的遗传多样性和基因型药物敏感性进行了首次调查。1998年至2003年期间，48名未接受过药物治疗的格鲁吉亚HIV阳性个体提供了外周血单核细胞（PBMC）DNA。基于部分pol序列的系统发育分析，主要的HIV-1基因形式是A亚型（70%），其次是B亚型（26%）；这两种基因形式在注射吸毒者和异性恋者中均有发现。还有1例C亚型（2%）和1例CRF18_cpx（2%）。格鲁吉亚A亚型毒株与来自俄罗斯的A亚型毒株聚类，命名为A(FSU)。12株A亚型毒株（25%）含有次要蛋白酶抑制剂突变V77I，其中9株还存在另外两个沉默突变。这种“V77I单倍型”标志着前苏联该流行病的一个特定遗传谱系。2株（4%）携带抗逆转录病毒（ARV）药物耐药突变。还完成了5株格鲁吉亚毒株的近乎全长的基因组序列。其中2株，98GEMZ011（A亚型）和98GEMZ003（B亚型），与重组产生CRF03_AB的亲本毒株非常相似。在分析中使用这些亲本毒株揭示了CRF03_AB中另外一段A亚型序列。因此，格鲁吉亚的HIV-1流行由A(FSU)亚型和B亚型混合组成。
