Rota A, Ström B, Linde-Forsberg C
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Box 7039, 750 07 Uppsala, Sweden.
Theriogenology. 1995 Oct 15;44(6):885-900. doi: 10.1016/0093-691x(95)00278-g.
Semen preservation and artificial insemination (AI) in the canine has become a common practice in veterinary medicine. Chilled dog semen is easy to handle, and several extenders can be used. The aim of this study was to compare the effects on canine spermatozoa of seminal plasma and 3 extenders commonly used for chilled semen preservation in clinical practice. The characteristics evaluated were sperm motility; velocity; plasma membrane status (assessed with a fluorescence staining technique and hypo-osmotic swelling test); acrosome morphology; semen pH; and semen osmolarity. These criteria were monitored daily in the ejaculates of 11 dogs. The ejaculates were divided into 4 aliquots. Each aliquot was extended in autologous seminal plasma, egg-yolk Tris, egg-yolk milk or egg-yolk cream and preserved at 4 degrees C for 4 d. In 10 of 11 semen samples extended in autologous seminal plasma, motility had already decreased to 0% by Day 2, and the percentage of spermatozoa with intact membranes was lower than in the 3 extenders (P < 0.05). Motility up to Day 4 was higher in egg-yolk Tris-stored spermatozoa (53.6%) than in those preserved in egg-yolk milk (30.4%) and egg-yolk cream (14.1%). Spermatozoa stored in egg-yolk Tris also had the highest sperm velocity, whereas no difference was found in plasma membrane or acrosome status (P>0.05). Egg-yolk Tris extender seems to be superior to the other extenders tested, to preserve dog semen at 4 degrees C, although differences were not significant for all the parameters.
犬精液保存和人工授精在兽医学中已成为一种常见的做法。冷冻犬精液易于处理，并且可以使用多种稀释液。本研究的目的是比较精浆和临床实践中常用于冷冻精液保存的3种稀释液对犬精子的影响。评估的特征包括精子活力、速度、质膜状态（通过荧光染色技术和低渗肿胀试验评估）、顶体形态、精液pH值和精液渗透压。每天对11只犬的射精样本进行这些指标的监测。射精样本被分成4份。每份样本分别用自体精浆、蛋黄Tris、蛋黄奶或蛋黄奶油进行稀释，并在4℃下保存4天。在11份用自体精浆稀释的精液样本中，有10份在第2天时活力已降至0%，并且具有完整膜的精子百分比低于其他3种稀释液（P<0.05）。到第4天时，用蛋黄Tris保存的精子活力（53.6%）高于用蛋黄奶（30.4%）和蛋黄奶油（14.1%）保存的精子。保存在蛋黄Tris中的精子也具有最高的精子速度，而在质膜或顶体状态方面未发现差异（P>0.05）。蛋黄Tris稀释液似乎优于所测试的其他稀释液，可在4℃下保存犬精液，尽管并非所有参数的差异都具有显著性。
