Hori Tatsuya, Yoshikuni Ryuta, Kobayashi Masanori, Kawakami Eiichi
Department of Reproduction, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
J Vet Med Sci. 2014 Mar 1;76(2):259-63. doi: 10.1292/jvms.13-0303. Epub 2013 Oct 1.
The objective of the present study was to determine an optimum temperature and extender for short-term transport of canine ejaculated semen. There was no significant difference in the qualities of semen diluted with two kinds of extender, egg yolk Tris-citrate fructose (EYT-FC) or glucose (EYT-GC) extender, between the 2, 8 or 12 and the 4°C control groups during storage for up to 48 hr, while the 16-24°C groups showed decreased sperm motility during storage for 48 hr. However, the 2°C group showed slightly lower sperm motility and slightly higher sperm abnormality than the 4°C group. Therefore, we concluded that semen qualities can be maintained for up to 48 hr when canine semen samples are extended with EYT-FC or EYT-GC and stored at a temperature in the range of 4-12°C.
本研究的目的是确定犬射精精液短期运输的最佳温度和稀释液。用两种稀释液（蛋黄柠檬酸三钠果糖（EYT-FC）或葡萄糖（EYT-GC）稀释液）稀释的精液，在储存长达48小时期间，2、8或12°C组与4°C对照组之间的精液质量没有显著差异，而16 - 24°C组在储存48小时期间精子活力下降。然而，2°C组的精子活力略低于4°C组，精子异常率略高于4°C组。因此，我们得出结论，当犬精液样本用EYT-FC或EYT-GC稀释并储存在4 - 12°C范围内的温度下时，精液质量可维持长达48小时。
