Okahashi N, Sasakawa C, Yoshikawa M, Hamada S, Koga T
Department of Dental Research, National Institute of Health, Tokyo, Japan.
Mol Microbiol. 1989 May;3(5):673-8. doi: 10.1111/j.1365-2958.1989.tb00215.x.
The complete nucleotide sequence of the gene for a cell-surface protein antigen (PAc) of Streptococcus mutans MT8148 (serotype c) was determined. The pac gene consisted of 4695 bp and coded for a 170773D protein. The pac gene product contained a putative 38 amino acid signal peptide, resulting in a 166817D mature protein. A potential promoter sequence and a putative Shine-Dalgarno sequence preceded the open reading frame. Two internal repeating amino acid sequences were present in the PAc. One repeating region located in the N-terminal region was rich in alanine, and the other located in the central region was rich in proline. Southern blot analysis under the less stringent condition (allowing up to 35% base mismatch) revealed that the probe covering the proline-rich region hybridized to DNA preparations from strains of Streptococcus cricetus, Streptococcus sobrinus and Streptococcus downei as well as Streptococcus mutans.
测定了变形链球菌MT8148（血清型c）细胞表面蛋白抗原（PAc）基因的完整核苷酸序列。pac基因由4695个碱基对组成，编码一种170773D的蛋白质。pac基因产物包含一个推定的38个氨基酸的信号肽，产生一个166817D的成熟蛋白质。在开放阅读框之前有一个潜在的启动子序列和一个推定的SD序列。PAc中存在两个内部重复氨基酸序列。一个位于N端区域的重复区域富含丙氨酸，另一个位于中央区域的重复区域富含脯氨酸。在较低严格条件下（允许高达35%的碱基错配）进行的Southern印迹分析表明，覆盖富含脯氨酸区域的探针与仓鼠链球菌、远缘链球菌、唐氏链球菌以及变形链球菌菌株的DNA制剂杂交。
