Mohr Harald, Bayer Anette, Gravemann Ute, Müller Thomas H
Blood Center of the German Red Cross Chapters of NSTOB, Institutes Springe and Gera, Springe, Germany.
Transfusion. 2006 Jun;46(6):949-55. doi: 10.1111/j.1537-2995.2006.00827.x.
The prevalence of bacterial contamination of random-donor platelet concentrates (PCs) is considerably lower than that of blood donations. Which key steps of the preparation procedure contribute to the elimination of bacteria was investigated.
Ten bacteria species were used. Blood donations were spiked with bacteria and stored at 22 degrees C for 8 hours. The buffy coats were kept for 6 hours. PCs were prepared from pools of 4 buffy coats. At each preparation step and during PC storage, bacteria contents were measured. In additional experiments, the titers of spiked blood and buffy coats were determined after storage at 20, 22, or 24 degrees C for 8 and up to 24 hours, respectively.
Enterobacter cloacae, Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, and Yersinia enterocolitica were completely inactivated during storage in blood or buffy coats. Titer reduction was between 3.32 and 4.62 log. Bacillus cereus, Propionibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis did not multiply. Compared with their values in spiked blood the titers in the PCs were reduced by 1.7 to 2.8 log. Klebsiella pneumoniae was the only species that grew in blood. With the exception of P. acnes, those species that were not removed by the preparation process multiplied in the PCs. Remarkable donor-to-donor variations of the bactericidal activities of buffy coats were detected when the storage time was prolonged to 24 hours.
Bacteria are significantly eliminated by the preparation procedure for random donor PCs. Also, blood and buffy coats are bactericidal for most species. When buffy-coat storage is prolonged, it cannot, however, be predicted whether specific strains vanish or multiply.
随机供者血小板浓缩物（PCs）的细菌污染率显著低于全血捐献。本研究调查了制备过程中的哪些关键步骤有助于消除细菌。
使用了10种细菌。将细菌加入全血捐献中，并在22℃下储存8小时。将白膜层保存6小时。从4个白膜层混合制备PCs。在每个制备步骤以及PCs储存期间，测量细菌含量。在额外的实验中，分别在20、22或24℃下储存8小时和长达24小时后，测定加入细菌的全血和白膜层的滴度。
阴沟肠杆菌、大肠埃希菌、铜绿假单胞菌、黏质沙雷菌和小肠结肠炎耶尔森菌在全血或白膜层储存期间完全失活。滴度降低了3.32至4.62个对数。蜡样芽孢杆菌、痤疮丙酸杆菌、金黄色葡萄球菌和表皮葡萄球菌没有增殖。与加入细菌的全血中的滴度相比，PCs中的滴度降低了1.7至2.8个对数。肺炎克雷伯菌是唯一在全血中生长的菌种。除痤疮丙酸杆菌外，那些未被制备过程去除的菌种在PCs中增殖。当储存时间延长至24小时时，检测到白膜层杀菌活性存在显著的供者间差异。
随机供者PCs的制备过程能显著消除细菌。此外，全血和白膜层对大多数菌种具有杀菌作用。然而，当白膜层储存时间延长时，无法预测特定菌株是消失还是增殖。
