Zanzotto A, Burruano S, Marciano P
C.R.A.-Istituto Sperimentale per la Viticoltura, Viale XXVIII aprile, 26-31015 Conegliano (TV), Italy.
Int J Food Microbiol. 2006 Jul 15;110(2):155-9. doi: 10.1016/j.ijfoodmicro.2006.04.003. Epub 2006 Jun 2.
Aspergillus strains belonging to the Aspergillus niger aggregate, either isolated from Italian grapes or received from public collections, were analysed in order to discriminate between the ochratoxin A (OTA) producing and the non-producing strains by means of the analysis of Internal Transcribed Spacers (ITS), Intergenic Spacers (IGS) and of a beta-tubulin gene portion. A. niger and Aspergillus awamori were identified observing the macro- and microscopic features of the colonies and the strains ochratoxigenicity was evaluated through Thin Layer Chromatography and/or High Performance Liquid Chromatography. PCR amplification of ITS, IGS and beta-tubulin gene portion produced 600, 440 and 550 bp amplicons, respectively, in all the analysed strains. The digestion of the IGS amplicon with Hinf I and of the other two amplicons with Rsa I, revealed one main profile type ("T-C-C") associated with the A. niger aggregate strains which lack the ability to produce OTA.
对从意大利葡萄中分离得到或从公共菌种保藏中心获取的属于黑曲霉复合体的曲霉菌株进行分析，以便通过分析内部转录间隔区（ITS）、基因间隔区（IGS）和β-微管蛋白基因片段，区分产赭曲霉毒素A（OTA）的菌株和不产该毒素的菌株。通过观察菌落的宏观和微观特征鉴定黑曲霉和泡盛曲霉，并通过薄层色谱法和/或高效液相色谱法评估菌株的产赭曲霉毒素能力。在所有分析菌株中，ITS、IGS和β-微管蛋白基因片段的PCR扩增分别产生了600、440和550 bp的扩增子。用Hinf I消化IGS扩增子，用Rsa I消化其他两个扩增子，结果显示一种主要的谱型（“T-C-C”）与缺乏产生OTA能力的黑曲霉复合体菌株相关。
