CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Stud Mycol. 2007;59:129-45. doi: 10.3114/sim.2007.59.13.
The present taxonomy of the black aspergilli reveals that there are 19 accepted taxa. However the identification of species of Aspergillus section Nigri is often problematic in spite of the existence of numerous methods proposed. An overview is provided of phenotypic and molecular methods to identify the accepted species of the black aspergilli. Colony morphology, conidial size and ornamentation of the ex type cultures is presented in a pictorial overview. The temperature range of all species is given and their growth characteristics on creatine agar and boscalid agar, a medium which was developed as a selective medium for the isolation of A. carbonarius are also shown. The extrolites produced by each species are listed while the response of the Ehrlich reaction is described. The literature on the various molecular methods to be used for species identification is reviewed and a critical evaluation of the usefulness of various techniques and genomic loci for species identification of black aspergilli is presented.
目前的黑曲霉分类学揭示出有 19 个被接受的分类单元。然而，尽管已经提出了许多方法，但是鉴定曲霉属 Nigri 组的物种仍然常常存在问题。本文概述了用于鉴定被接受的黑曲霉物种的表型和分子方法。通过图片概览展示了各模式种的菌落形态、分生孢子大小和纹饰。给出了所有物种的温度范围，并展示了它们在精氨酸琼脂和 boscalid 琼脂上的生长特性，后者是作为分离 A. carbonarius 的选择性培养基而开发的。列出了每个物种产生的外生代谢产物，同时还描述了 Ehrlich 反应的情况。综述了用于物种鉴定的各种分子方法的文献，并对黑曲霉物种鉴定中各种技术和基因组基因座的有用性进行了批判性评估。
