Bau M, Castellá G, Bragulat M R, Cabañes F J
Grup de Micologia Veterinària, Departament de Sanitat i d'Anatomia Animals, Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
Int J Food Microbiol. 2006 Sep 1;111 Suppl 1:S18-21. doi: 10.1016/j.ijfoodmicro.2006.03.008. Epub 2006 May 11.
In order to characterize by molecular methods the Aspergillus niger aggregate species involved in the ochratoxin A (OTA) contamination of European wine grapes and table grapes from Israel, a total of 173 strains were studied. The ITS-5.8S rDNA fragments of 173 A. niger agreggate strains from grapes included in this study were amplified and their PCR amplicons were RsaI digested in order to classify the strains in the RFLP types, N and T. All of the strains belonging to the A. niger aggregate were classified into the two RFLP types previously defined: type N (43%) and type T (57%). Twenty out of the 173 strains of A. niger aggregate produced OTA (0.1 to 10.5 mug g(-1)). All the OTA producing species belonged to the N-RFLP type.
为了通过分子方法鉴定参与欧洲酿酒葡萄和以色列鲜食葡萄赭曲霉毒素A（OTA）污染的黑曲霉聚集种，共研究了173株菌株。对本研究中葡萄来源的173株黑曲霉聚集菌株的ITS-5.8S rDNA片段进行扩增，并对其PCR扩增产物进行RsaI酶切，以便将菌株分类为RFLP类型N和T。所有属于黑曲霉聚集种的菌株都被分类为先前定义的两种RFLP类型：N型（43%）和T型（57%）。173株黑曲霉聚集菌株中有20株产生OTA（0.1至10.5μg g-1）。所有产生OTA的菌种都属于N-RFLP类型。
