Yoon Jung-Hoon, Kang So-Jung, Oh Hyun Woo, Oh Tae-Kwang
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Int J Syst Evol Microbiol. 2006 Jun;56(Pt 6):1363-1367. doi: 10.1099/ijs.0.64091-0.
A Gram-negative, rod-shaped, Stenotrophomonas-like bacterial strain, DS-16(T), was isolated from soil from Dokdo, Korea, and subjected to a polyphasic taxonomic study. Strain DS-16(T) grew optimally at pH 6.0-7.0 and 30 degrees C in the presence of 0.5 % (w/v) NaCl. It contained Q-8 as the predominant ubiquinone and iso-C(16 : 0), iso-C(15 : 0) and iso-C(17 : 1)omega9c as the major fatty acids. The DNA G + C content was 65.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DS-16(T) joined the cluster comprising Stenotrophomonas species. The levels of 16S rRNA gene sequence similarity between strain DS-16(T) and the type strains of Stenotrophomonas species ranged from 95.5 to 97.5 %. DNA-DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness of strain DS-16(T), demonstrated that this novel strain differs from Stenotrophomonas species with validly published names. On the basis of phenotypic, phylogenetic and genetic data, strain DS-16(T) (=KCTC 12543(T) = CIP 108839(T)) should be classified in the genus Stenotrophomonas as a member of a novel species, for which the name Stenotrophomonas dokdonensis sp. nov. is proposed.
从韩国独岛的土壤中分离出一株革兰氏阴性、杆状、类似嗜麦芽窄食单胞菌的细菌菌株DS-16(T)，并对其进行了多相分类研究。菌株DS-16(T)在pH 6.0 - 7.0和30℃、0.5%（w/v）NaCl存在的条件下生长最佳。它含有Q-8作为主要泛醌，以及异-C(16 : 0)、异-C(15 : 0)和异-C(17 : 1)ω9c作为主要脂肪酸。DNA G + C含量为65.1 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株DS-16(T)加入了包含嗜麦芽窄食单胞菌属物种的簇。菌株DS-16(T)与嗜麦芽窄食单胞菌属物种模式菌株之间的16S rRNA基因序列相似性水平在95.5%至97.5%之间。DNA - DNA相关性数据和差异表型特征，以及菌株DS-16(T)的系统发育独特性，表明该新菌株与已有效发表名称的嗜麦芽窄食单胞菌属物种不同。基于表型、系统发育和遗传数据，菌株DS-16(T)（=KCTC 12543(T) = CIP 108839(T)）应归类于嗜麦芽窄食单胞菌属，作为一个新物种的成员，为此提议将其命名为嗜麦芽窄食单胞菌独岛新种（Stenotrophomonas dokdonensis sp. nov.）。
