Insect Resistance Group, International Centre for Genetic Engineering and Biotechnology, ICGEB Campus, Aruna Asaf Ali Marg, New Delhi 110067, India.
J Microbiol. 2010 Oct;48(5):601-6. doi: 10.1007/s12275-010-0125-7. Epub 2010 Nov 3.
A Gram-negative, aerobic, golden yellow, rod-shaped bacterium, a strain designated ICGEB-L15(T), was isolated from the larval midgut of Anopheles stephensi captured in District Jhajjar, Haryana, India. The strain ICGEB-L15(T) grows at 30-50°C (optimum 30-37°C), pH 6.5-8.5 (optimum 7.0-8.0) and in the presence of 2% NaCl. The major fatty acids were iso-C(15:0) (22.5% of total fatty acid), anteiso-C(15:0) (16.5%), iso-C(17:1) 9c (10.3%), iso-C(16:0) (7.3%), C(16:0) (6.1%), and iso-C(11:0) (5.3%). The strain showed the highest 16S rRNA gene sequence similarities with the type strains Pseudoxanthomonas daejeonensis KCTC 12207(T) (97.4%), Pseudoxanthomonas kaohsiungensis J36(T) (97.17%), and Pseudoxanthomonas mexicana AMX 26B(T) (97.11%). The DNA relatedness between ICGEB-L15(T) and Pseudoxanthomonas daejeonensis KCTC 12207(T), Pseudoxanthomonas kaohsiungensis J36(T) and Pseudoxanthomonas mexicana AMX 26B(T) was 24.5%, 28.2%, and 33.6%, respectively. The G+C content of genomic DNA was 69.9 mol%. The major isoprenoid quinone of strain ICGEB-L15(T) was Q-8. The strain ICGEB-L15(T) represents a novel species of the genus Pseudoxanthomonas based on physiological, biochemical and phylogenetic properties; therefore, the name Pseudoxanthomonas icgebensis sp. nov. is proposed. The type strain is ICGEB-L15(T) (=KACC 14090(T) =DSM 22536(T)).
一株革兰氏阴性、需氧、金黄色、杆状细菌，被命名为 ICGEB-L15(T)，是从印度哈里亚纳邦 Jhajjar 区捕获的致倦库蚊幼虫中分离得到的。菌株 ICGEB-L15(T) 在 30-50°C（最佳 30-37°C）、pH6.5-8.5（最佳 7.0-8.0）和 2%NaCl 的条件下生长。主要脂肪酸为 iso-C(15:0)（22.5%总脂肪酸）、anteiso-C(15:0)（16.5%）、iso-C(17:1) 9c（10.3%）、iso-C(16:0)（7.3%）、C(16:0)（6.1%）和 iso-C(11:0)（5.3%）。该菌株与模式菌株 Pseudoxanthomonas daejeonensis KCTC 12207(T)（97.4%）、Pseudoxanthomonas kaohsiungensis J36(T)（97.17%）和 Pseudoxanthomonas mexicana AMX 26B(T)（97.11%）的 16S rRNA 基因序列相似度最高。ICGEB-L15(T)与 Pseudoxanthomonas daejeonensis KCTC 12207(T)、Pseudoxanthomonas kaohsiungensis J36(T)和 Pseudoxanthomonas mexicana AMX 26B(T)的 DNA 相关度分别为 24.5%、28.2%和 33.6%。基因组 DNA 的 G+C 含量为 69.9mol%。菌株 ICGEB-L15(T)的主要类异戊二烯醌为 Q-8。根据生理、生化和系统发育特性，菌株 ICGEB-L15(T)代表了假单胞菌属的一个新种，因此提出了 Pseudoxanthomonas icgebensis sp. nov.的名称。模式菌株为 ICGEB-L15(T)=KACC 14090(T)=DSM 22536(T)。
