Appendix: Analysis of pH-dependent kinetics in up to four reactive hydronic states.
作者信息
Brocklehurst S M, Brocklehurst K
机构信息
Department of Chemistry, University of York, Heslington, York, U.K.
出版信息
Biochem J. 1988 Dec 1;256(2):556-8. doi: 10.1042/bj2560556.
Abstract
摘要
相似文献
1
Appendix: Analysis of pH-dependent kinetics in up to four reactive hydronic states.附录：多达四种反应性水合状态下pH依赖性动力学分析
Biochem J. 1988 Dec 1;256(2):556-8. doi: 10.1042/bj2560556.
2
Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics.利用天然结构变异体和反应性探针动力学对有助于酶催化的相互作用进行超晶体学分辨率研究。
Biochem J. 1988 Dec 1;256(2):543-58. doi: 10.1042/bj2560543.
3
Structure-function relationships in the cysteine proteinases actinidin, papain and papaya proteinase omega. Three-dimensional structure of papaya proteinase omega deduced by knowledge-based modelling and active-centre characteristics determined by two-hydronic-state reactivity probe kinetics and kinetics of catalysis.半胱氨酸蛋白酶肌动蛋白水解酶、木瓜蛋白酶和木瓜蛋白酶ω的结构-功能关系。通过基于知识的建模推导木瓜蛋白酶ω的三维结构，以及通过双水合态反应探针动力学和催化动力学确定其活性中心特征。
Biochem J. 1991 Nov 15;280 ( Pt 1)(Pt 1):79-92. doi: 10.1042/bj2800079.
4
Catalysis by acetylcholinesterase in two-hydronic-reactive states. Integrity of deuterium oxide effects and hydron inventories.乙酰胆碱酯酶在两种水合反应状态下的催化作用。重水效应和氢库存的完整性。
Biochem J. 1992 Jul 15;285 ( Pt 2)(Pt 2):451-60. doi: 10.1042/bj2850451.
5
Clarification of the pH-dependent kinetic behaviour of papain by using reactivity probes and analysis of alkylation and catalysed acylation reactions in terms of multihydronic state models: implications for electrostatics calculations and interpretation of the consequences of site-specific mutations such as Asp-158-Asn and Asp-158-Glu.利用反应性探针阐明木瓜蛋白酶的pH依赖性动力学行为，并根据多质子态模型分析烷基化和催化酰化反应：对静电计算以及对位点特异性突变（如Asp-158-Asn和Asp-158-Glu）后果的解释的意义。
Biochem J. 1993 Aug 15;294 ( Pt 1)(Pt 1):201-10. doi: 10.1042/bj2940201.
6
Catalytic-site characteristics of the porcine calpain II 80 kDa/18 kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors: evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group.通过猪钙蛋白酶II 80 kDa/18 kDa异二聚体的必需巯基与双水合态时间依赖性抑制剂的选择性反应揭示的催化位点特征：催化位点半胱氨酸/组氨酸相互作用系统和电离调节基团的证据。
Biochem J. 1993 Feb 15;290 ( Pt 1)(Pt 1):75-83. doi: 10.1042/bj2900075.
7
Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.糜蛋白酶M的结构：通过比较建模技术推导得出的晚期洗脱糜蛋白酶，以及通过pH依赖性催化动力学和与时间依赖性抑制剂反应确定的活性中心特征：半胱氨酸-25/组氨酸-159离子对对于糜蛋白酶M和木瓜蛋白酶的催化活性而言均不充分。
Biochem J. 1994 Jun 15;300 ( Pt 3)(Pt 3):805-20. doi: 10.1042/bj3000805.
8
The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain.静电场与结合相互作用的相互影响决定了猕猴桃蛋白酶催化位点的反应活性。猕猴桃蛋白酶和木瓜蛋白酶行为差异的一个可能来源。
Biochem J. 1989 Apr 15;259(2):443-52. doi: 10.1042/bj2590443.
9
Testing of the bactericidal action of products based on the "hydronic" technology ("informed glass") on ATCC strains of pathogenic Gram-positive and Gram-negative bacteria and yeasts (Staphylococcus aureus, Escherichia coli, and Candida albicans).基于“水力”技术（“知情玻璃”）的产品对 ATCC 致病性革兰氏阳性和革兰氏阴性细菌和酵母（金黄色葡萄球菌、大肠杆菌和白色念珠菌）的杀菌作用测试。
J Altern Complement Med. 2010 Apr;16(4):463-71. doi: 10.1089/acm.2009.0600.
10
[Influence of hydrone on the dental pulp as compared to other drugs].
Polim Med. 1978;8(2):65-79.
引用本文的文献
1
Catalytic-site characteristics of the porcine calpain II 80 kDa/18 kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors: evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group.通过猪钙蛋白酶II 80 kDa/18 kDa异二聚体的必需巯基与双水合态时间依赖性抑制剂的选择性反应揭示的催化位点特征：催化位点半胱氨酸/组氨酸相互作用系统和电离调节基团的证据。
Biochem J. 1993 Feb 15;290 ( Pt 1)(Pt 1):75-83. doi: 10.1042/bj2900075.
2
Clarification of the pH-dependent kinetic behaviour of papain by using reactivity probes and analysis of alkylation and catalysed acylation reactions in terms of multihydronic state models: implications for electrostatics calculations and interpretation of the consequences of site-specific mutations such as Asp-158-Asn and Asp-158-Glu.利用反应性探针阐明木瓜蛋白酶的pH依赖性动力学行为，并根据多质子态模型分析烷基化和催化酰化反应：对静电计算以及对位点特异性突变（如Asp-158-Asn和Asp-158-Glu）后果的解释的意义。
Biochem J. 1993 Aug 15;294 ( Pt 1)(Pt 1):201-10. doi: 10.1042/bj2940201.
3
Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.糜蛋白酶M的结构：通过比较建模技术推导得出的晚期洗脱糜蛋白酶，以及通过pH依赖性催化动力学和与时间依赖性抑制剂反应确定的活性中心特征：半胱氨酸-25/组氨酸-159离子对对于糜蛋白酶M和木瓜蛋白酶的催化活性而言均不充分。
Biochem J. 1994 Jun 15;300 ( Pt 3)(Pt 3):805-20. doi: 10.1042/bj3000805.
4
Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics.利用天然结构变异体和反应性探针动力学对有助于酶催化的相互作用进行超晶体学分辨率研究。
Biochem J. 1988 Dec 1;256(2):543-58. doi: 10.1042/bj2560543.
5
Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papain.确定信号和非信号结合对酶反应性的贡献。结合相互作用的不同组合导致木瓜蛋白酶反应中过渡态几何结构的变化。
Biochem J. 1989 Mar 15;258(3):755-64. doi: 10.1042/bj2580755.
6
The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain.静电场与结合相互作用的相互影响决定了猕猴桃蛋白酶催化位点的反应活性。猕猴桃蛋白酶和木瓜蛋白酶行为差异的一个可能来源。
Biochem J. 1989 Apr 15;259(2):443-52. doi: 10.1042/bj2590443.
7
A re-appraisal of the structural basis of stereochemical recognition in papain. Insensitivity of binding-site-catalytic-site signalling to P2-chirality in a time-dependent inhibition.木瓜蛋白酶中立体化学识别结构基础的重新评估。在时间依赖性抑制中，结合位点 - 催化位点信号对P2手性不敏感。
Biochem J. 1990 Mar 15;266(3):645-51. doi: 10.1042/bj2660645.
8
A polyclonal antibody preparation with Michaelian catalytic properties.一种具有米氏催化特性的多克隆抗体制剂。
Biochem J. 1991 Nov 1;279 ( Pt 3)(Pt 3):871-81. doi: 10.1042/bj2790871.
本文引用的文献
1
Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics.利用天然结构变异体和反应性探针动力学对有助于酶催化的相互作用进行超晶体学分辨率研究。
Biochem J. 1988 Dec 1;256(2):543-58. doi: 10.1042/bj2560543.
2
Consequences of molecular recognition in the S1-S2 intersubsite region of papain for catalytic-site chemistry. Change in pH-dependence characteristics and generation of an inverse solvent kinetic isotope effect by introduction of a P1-P2 amide bond into a two-protonic-state reactivity probe.木瓜蛋白酶S1 - S2亚位点间区域分子识别对催化位点化学的影响。通过将P1 - P2酰胺键引入双质子态反应性探针，pH依赖性特征的变化及反向溶剂动力学同位素效应的产生。
Biochem J. 1988 Mar 15;250(3):761-72. doi: 10.1042/bj2500761.
3
pH-activity curves for enzyme-catalysed reactions in which the hydron is a product or reactant.质子作为产物或反应物的酶催化反应的pH-活性曲线。
Biochem J. 1987 Dec 1;248(2):573-8. doi: 10.1042/bj2480573.
4
The equilibrium assumption is valid for the kinetic treatment of most time-dependent protein-modification reactions.平衡假设对于大多数随时间变化的蛋白质修饰反应的动力学处理是有效的。
Biochem J. 1979 Sep 1;181(3):775-8. doi: 10.1042/bj1810775.
5
Effects of pH on enzymes.pH对酶的影响。
Methods Enzymol. 1979;63:183-234. doi: 10.1016/0076-6879(79)63011-2.
6
Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system.使用双质子态亲电试剂作为反应性探针表征木瓜蛋白酶活性中心。在不间断的半胱氨酸-25-组氨酸-159相互作用系统中亲核反应性的证据。
Biochem J. 1978 May 1;171(2):385-401. doi: 10.1042/bj1710385.
7
The pH-dependence of second-order rate constants of enzyme modification may provide free-reactant pKa values.酶修饰二级速率常数的pH依赖性可提供游离反应物的pKa值。
Biochem J. 1977 Dec 1;167(3):859-62. doi: 10.1042/bj1670859.
8
9
PH-dependence of the steady-state rate of a two-step enzymic reaction.两步酶促反应稳态速率的pH依赖性
Biochem J. 1976 Apr 1;155(1):61-70. doi: 10.1042/bj1550061.
Zotero 插件