吡啶血色原的形成。
The formation of pyridine haemochromogen.
作者信息
Gallagher W A, Elliott W B
机构信息
Department of Biochemistry, State University of New York at Buffalo, Buffalo, N.Y. 14214, U.S.A.
出版信息
Biochem J. 1965 Oct;97(1):187-93. doi: 10.1042/bj0970187.
Abstract
- Titration of haem with pyridine in alkaline media of low ionic strength yields a true pyridine haemochromogen, compound III, at very low concentrations of pyridine. 2. Graphical analysis of this titration gives the first spectrophotometric evidence for a dimeric haem. 3. Compound III is unstable and tends to aggregate to a second compound, compound II, whose formation is enhanced under those conditions favourable to hydrophobic bonding. 4. At higher concentrations of pyridine, compound II is dispersed to yield the classical pyridine haemochromogen, compound I, whose spectral properties are essentially those of pyridine haemochromogen in a non-aqueous medium.
摘要
- 在低离子强度的碱性介质中，用吡啶滴定血红素，在吡啶浓度非常低时会产生真正的吡啶血色原，即化合物III。2. 该滴定的图形分析为二聚体血红素提供了首个分光光度法证据。3. 化合物III不稳定，倾向于聚合成第二种化合物，即化合物II，在有利于疏水键合的条件下其形成会增强。4. 在较高吡啶浓度下，化合物II会分散形成经典的吡啶血色原，即化合物I，其光谱特性基本上与非水介质中的吡啶血色原相同。
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