Murthy Papolu Bhargava Sriramachandra, Raju Valivarti Raghava, Ramakrisana Tummala, Chakravarthy Mangu Srinivasa, Kumar Katta Vijay, Kannababu Sukala, Subbaraju Gottumukkala Venkata
Laila Research Centre, Unit I, Phase III, Jawahar Autonagar, Vijayawada-520 007, India.
Chem Pharm Bull (Tokyo). 2006 Jun;54(6):907-11. doi: 10.1248/cpb.54.907.
A simple and sensitive reversed phase high performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of twelve bacopa saponins present in the extracts of the Indian Medicinal Plant, Bacopa monnieri. The separation was achieved on a reversed phase C(18) column (Luna C(18)), 5 microm by isocratic elution with 0.05 M sodium sulphate buffer (pH 2.3) and acetonitrile (68.5 : 31.5, v/v) as the mobile phase at a flow rate of 1.0 ml/min with an operating temperature of 30 degrees C. The method was validated for linearity, precision, intra- and inter-day precision and accuracy. Several Bacopa samples (plant materials, extracts and commercial formulations) were successfully analyzed. Major bacopasaponins were bacosides A(3) (3), bacopaside II (4), bacopaside I (5), bacopaside X (6), bacopasaponin C (7), bacopaside N2 (9) and the minor components were bacopasaponin F (1), bacopasaponin E (2), bacopaside N1 (8) bacopaside III (10), bacopaside IV (11) and bacopaside V (12). The total saponin content in the samples, plant materials and extracts varied from 5.1 to 22.17% and 1.47 to 66.03 mg/capsule or tablet in the commercial formulations.
已开发出一种简单且灵敏的反相高效液相色谱（HPLC）方法，用于同时测定印度药用植物假马齿苋提取物中存在的十二种假马齿苋皂苷。分离在反相C(18)柱（月旭C(18)）上进行，柱长5微米，以0.05M硫酸钠缓冲液（pH 2.3）和乙腈（68.5:31.5，v/v）作为流动相进行等度洗脱，流速为1.0 ml/min，操作温度为30℃。该方法针对线性、精密度、日内和日间精密度以及准确度进行了验证。成功分析了多个假马齿苋样品（植物材料、提取物和商业制剂）。主要的假马齿苋皂苷有假马齿苋皂苷A(3)（3）、假马齿苋皂苷II（4）、假马齿苋皂苷I（5）、假马齿苋皂苷X（6）、假马齿苋皂苷C（7）、假马齿苋皂苷N2（9），次要成分有假马齿苋皂苷F（1）、假马齿苋皂苷E（2）、假马齿苋皂苷N1（8）、假马齿苋皂苷III（10）、假马齿苋皂苷IV（11）和假马齿苋皂苷V（12）。样品、植物材料和提取物中的总皂苷含量在5.1%至22.17%之间，商业制剂中的含量为1.47至66.03毫克/胶囊或片剂。
