Department of Bromatology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, Mickiewicza 2D, 15-222, Białystok, Poland.
Department of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, Mickiewicza 2a, 15-230, Białystok, Poland.
Sci Rep. 2020 Dec 3;10(1):21127. doi: 10.1038/s41598-020-78014-w.
Propolis and Bacopa monnieri (L.) Wettst. (Brahmi) are natural products that contain many active substances and possess anticancer properties. The aim of this study was to investigate the chemical composition of Polish propolis extract (PPE) by gas chromatography-mass spectrometry (GC-MS), B. monnieri extracts (BcH, BcS) by high performance liquid chromatography with diode array detector and mass spectrometry coupled with electrospray ionization (LC-ESI-MS) and finally determine its anti-proliferative potential combined with BcH and BcS in glioblastoma cell lines (T98G, LN-18, U87MG). The antiproliferative activity of PPE, BcH, BcS and their combination (PPE + BcH) was determined by a cytotoxicity test, and DNA binding was determined by [H]-thymidine incorporation. Flavonoids and phenylpropenoids were the main components of PPE. BcH and BcS samples were also successfully analyzed. Their main constituents were saponins such as bacoside A3, bacopaside II, X and bacopasaponin C and its isomer. The inhibitory effects on the viability and proliferation of the tested glioma cells observed after incubation with the combination of PPE and BcH were significantly stronger than the effects of these two extracts separately. These findings suggest that propolis in combination with B. monnieri shows promising anticancer activity for the treatment of glioblastoma. However, further studies are still required.
蜂胶和益智（L.） Wettst.（ Brahmi ）是天然产物，含有许多活性物质，具有抗癌特性。本研究的目的是通过气相色谱-质谱联用（GC-MS）分析波兰蜂胶提取物（PPE）的化学成分，通过高效液相色谱-二极管阵列检测器和质谱联用（LC-ESI-MS）分析益智提取物（BcH、BcS），最后确定其与益智提取物（BcH、BcS）联合使用的抗增殖潜力在神经胶质瘤细胞系（T98G、LN-18、U87MG）中。通过细胞毒性试验测定 PPE、BcH、BcS 及其组合（PPE+BcH）的抗增殖活性，并通过[H]-胸苷掺入测定 DNA 结合。黄酮类化合物和苯丙素类化合物是 PPE 的主要成分。BcH 和 BcS 样品也成功进行了分析。它们的主要成分是皂苷，如 bacoside A3、bacopaside II、X 和 bacopasaponin C 及其异构体。与 PPE 和 BcH 组合孵育后观察到对测试神经胶质瘤细胞活力和增殖的抑制作用明显强于这两种提取物单独作用的效果。这些发现表明，蜂胶与益智联合使用显示出治疗神经胶质瘤的有希望的抗癌活性。然而，仍需要进一步的研究。
