Pignone Michelle, Greth Kimberly M, Cooper Jason, Emerson David, Tang Jane
Bacteriology Program, American Type Culture Collection, 10801 University Blvd., Manassas, Virginia 20110, USA.
J Clin Microbiol. 2006 Jun;44(6):1963-70. doi: 10.1128/JCM.01959-05.
Classical methods for identification of Mycobacterium species rely on morphology and biochemical profiles. Speciation of a Mycobacterium isolate using these standard methods is a lengthy process based on subjective data interpretation. In this study, Mycobacterium species were characterized by utilizing matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). This technology is designed to provide a characteristic mass spectral fingerprint based on desorbed ions from the cell surface. Thirty-seven strains were analyzed; these represented thirteen species and five subspecies that included the Mycobacterium tuberculosis complex and the M. avium-M. intracellulare complex, as well as rapid- and slow-growing mycobacteria. All 37 strains were analyzed in triplicate, and a database was generated. This method produced species-specific patterns for all but 1 of the 37 isolates and provided reliable differentiation at the strain level. The data suggest that whole-cell MALDI-TOF MS has potential as a rapid and reproducible method for the identification and characterization of Mycobacterium species.
鉴定分枝杆菌属菌种的传统方法依赖于形态学和生化特征。使用这些标准方法对分枝杆菌分离株进行菌种鉴定是一个基于主观数据解读的漫长过程。在本研究中，利用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对分枝杆菌属菌种进行了特征分析。该技术旨在根据从细胞表面解吸的离子提供特征性的质谱指纹图谱。分析了37株菌株；它们代表了13个菌种和5个亚种，包括结核分枝杆菌复合群和鸟分枝杆菌-胞内分枝杆菌复合群，以及快速生长和缓慢生长的分枝杆菌。对所有37株菌株进行了三次重复分析，并建立了一个数据库。该方法为37株分离株中除1株外的所有菌株产生了种特异性图谱，并在菌株水平上提供了可靠的鉴别。数据表明，全细胞MALDI-TOF MS有潜力作为一种快速且可重复的方法用于分枝杆菌属菌种的鉴定和特征分析。
