Be aware of the possibility of false-positive results in single-locus PCR assays for methicillin-resistant Staphylococcus aureus.
作者信息
Rupp Jan, Fenner Ines, Solbach Werner, Gieffers Jens
出版信息
J Clin Microbiol. 2006 Jun;44(6):2317. doi: 10.1128/JCM.00490-06.
Abstract
摘要
相似文献
1
Be aware of the possibility of false-positive results in single-locus PCR assays for methicillin-resistant Staphylococcus aureus.注意耐甲氧西林金黄色葡萄球菌单基因座聚合酶链反应检测中出现假阳性结果的可能性。
J Clin Microbiol. 2006 Jun;44(6):2317. doi: 10.1128/JCM.00490-06.
2
Does nasal cocolonization by methicillin-resistant coagulase-negative staphylococci and methicillin-susceptible Staphylococcus aureus strains occur frequently enough to represent a risk of false-positive methicillin-resistant S. aureus determinations by molecular methods?耐甲氧西林凝固酶阴性葡萄球菌和甲氧西林敏感金黄色葡萄球菌菌株的鼻腔共同定植是否频繁发生，足以构成通过分子方法测定耐甲氧西林金黄色葡萄球菌出现假阳性结果的风险？
J Clin Microbiol. 2006 Jan;44(1):229-31. doi: 10.1128/JCM.44.1.229-231.2006.
3
The prevalence of methicillin-resistant staphylococci in healthy horses in the Netherlands.荷兰健康马匹中耐甲氧西林葡萄球菌的流行情况。
Vet Microbiol. 2006 Mar 10;113(1-2):131-6. doi: 10.1016/j.vetmic.2005.10.028. Epub 2005 Nov 21.
4
Improved and rapid detection of methicillin-resistant Staphylococcus aureus nasal carriage using selective broth and multiplex PCR.使用选择性肉汤和多重聚合酶链反应改进并快速检测耐甲氧西林金黄色葡萄球菌鼻腔定植情况
Res Microbiol. 2006 Dec;157(10):971-5. doi: 10.1016/j.resmic.2006.08.004. Epub 2006 Sep 12.
5
Detection of methicillin- and aminoglycoside-resistant genes and simultaneous identification of S. aureus using triplex real-time PCR Taqman assay.采用三重实时荧光定量PCR Taqman分析法检测耐甲氧西林和氨基糖苷类基因并同时鉴定金黄色葡萄球菌。
J Microbiol Methods. 2007 Jan;68(1):157-62. doi: 10.1016/j.mimet.2006.07.008. Epub 2006 Aug 28.
6
Decrease in the incidence of mupirocin resistance among methicillin-resistant Staphylococcus aureus in carriers from an intensive care unit.重症监护病房携带者中耐甲氧西林金黄色葡萄球菌对莫匹罗星耐药率的下降。
Am J Infect Control. 2006 Feb;34(1):6-9. doi: 10.1016/j.ajic.2005.08.006.
7
A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I-V.一种用于简便筛查I-V型耐甲氧西林金黄色葡萄球菌SCCmec的新型多重PCR方法。
Clin Microbiol Infect. 2007 Jul;13(7):725-7. doi: 10.1111/j.1469-0691.2007.01720.x. Epub 2007 Apr 2.
8
Clinical isolates of Staphylococcus intermedius masquerading as methicillin-resistant Staphylococcus aureus.伪装成耐甲氧西林金黄色葡萄球菌的中间葡萄球菌临床分离株。
J Clin Microbiol. 2004 Dec;42(12):5881-4. doi: 10.1128/JCM.42.12.5881-5884.2004.
9
[Drug-resistance of staphylococcus aureus and detection of mecA gene in all strains isolated from children in Hangzhou].[杭州地区儿童分离株金黄色葡萄球菌耐药性及mecA基因检测]
Zhonghua Er Ke Za Zhi. 2006 May;44(5):360-3.
10
Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus.三种用于快速鉴定耐甲氧西林金黄色葡萄球菌的分子检测方法的评估
J Clin Microbiol. 2007 Jun;45(6):2011-3. doi: 10.1128/JCM.00232-07. Epub 2007 Apr 11.
引用本文的文献
1
Detection of Methicillin-Resistant Infections Using Molecular Methods.使用分子方法检测耐甲氧西林感染
Antibiotics (Basel). 2022 Feb 12;11(2):239. doi: 10.3390/antibiotics11020239.
2
Detection of epidemic USA300 community-associated methicillin-resistant Staphylococcus aureus strains by use of a single allele-specific PCR assay targeting a novel polymorphism of Staphylococcus aureus pbp3.应用针对金黄色葡萄球菌 pbp3 新型多态性的单等位基因特异性 PCR 检测方法检测流行 USA300 社区相关耐甲氧西林金黄色葡萄球菌菌株。
J Clin Microbiol. 2013 Aug;51(8):2541-50. doi: 10.1128/JCM.00417-13. Epub 2013 May 22.
3
Rapid detection of Staphylococcus aureus in lower respiratory tract secretions from patients with suspected ventilator-associated pneumonia: evaluation of the Cepheid Xpert MRSA/SA SSTI assay.快速检测疑似呼吸机相关性肺炎患者下呼吸道分泌物中的金黄色葡萄球菌：Cepheid Xpert MRSA/SA SSTI 检测方法的评估。
J Clin Microbiol. 2012 Dec;50(12):4095-7. doi: 10.1128/JCM.02409-12. Epub 2012 Sep 19.
4
Detection of methicillin-resistant Staphylococcus aureus using double duplex real-time PCR and dye Syto 9.采用双重实时荧光 PCR 和染料 Syto 9 检测耐甲氧西林金黄色葡萄球菌。
Folia Microbiol (Praha). 2010 Sep;55(5):502-7. doi: 10.1007/s12223-010-0083-9. Epub 2010 Oct 13.
5
False-negative test results in the Slidex Staph Plus (bioMérieux) agglutination test are mainly caused by spa-type t001 and t001-related strains.Slidex Staph Plus（生物梅里埃）凝聚试验中的假阴性试验结果主要由 spa 型 t001 和 t001 相关菌株引起。
Eur J Clin Microbiol Infect Dis. 2011 Feb;30(2):201-8. doi: 10.1007/s10096-010-1070-4. Epub 2010 Oct 9.
6
Evaluation of the Xpert MRSA assay for rapid detection of methicillin-resistant Staphylococcus aureus from nares swabs of geriatric hospitalized patients and failure to detect a specific SCCmec type IV variant.评估 Xpert MRSA 检测法对老年住院患者鼻腔拭子中耐甲氧西林金黄色葡萄球菌的快速检测及对一种特定 SCCmec Ⅳ型变体漏检的情况。
Eur J Clin Microbiol Infect Dis. 2010 Aug;29(8):995-1002. doi: 10.1007/s10096-010-0958-3. Epub 2010 May 29.
7
Evaluation of single and double-locus real-time PCR assays for methicillin-resistant Staphylococcus aureus (MRSA) surveillance.用于耐甲氧西林金黄色葡萄球菌（MRSA）监测的单基因座和双基因座实时PCR检测方法的评估
BMC Res Notes. 2010 Apr 21;3:110. doi: 10.1186/1756-0500-3-110.
8
Contribution of two molecular assays as compared to selective culture for MRSA screening in a low MRSA prevalence population.两种分子检测方法与选择性培养相比在低耐甲氧西林金黄色葡萄球菌（MRSA）流行率人群中的 MRSA 筛查效果。
Infection. 2010 Apr;38(2):98-101. doi: 10.1007/s15010-009-9117-0. Epub 2010 Feb 27.
9
Clinical application of real-time PCR to screening critically ill and emergency-care surgical patients for methicillin-resistant Staphylococcus aureus: a quantitative analytical study.实时聚合酶链反应在筛选危重病和急诊手术患者耐甲氧西林金黄色葡萄球菌中的临床应用：一项定量分析研究。
J Clin Microbiol. 2009 Dec;47(12):4102-8. doi: 10.1128/JCM.01332-09. Epub 2009 Oct 21.
10
Detection of staphylococcal cassette chromosome mec-associated DNA segments in multiresistant methicillin-susceptible Staphylococcus aureus (MSSA) and identification of Staphylococcus epidermidis ccrAB4 in both methicillin-resistant S. aureus and MSSA.在多重耐药的甲氧西林敏感金黄色葡萄球菌（MSSA）中检测葡萄球菌盒式染色体mec相关DNA片段，并在耐甲氧西林金黄色葡萄球菌和MSSA中鉴定表皮葡萄球菌ccrAB4。
Antimicrob Agents Chemother. 2008 Dec;52(12):4407-19. doi: 10.1128/AAC.00447-08. Epub 2008 Oct 13.
本文引用的文献
1
PCR for the identification of methicillin-resistant Staphylococcus aureus (MRSA) strains using a single primer pair specific for SCCmec elements and the neighbouring chromosome-borne orfX.使用一对针对SCCmec元件及相邻染色体携带的orfX的特异性引物，通过聚合酶链反应（PCR）鉴定耐甲氧西林金黄色葡萄球菌（MRSA）菌株。
Clin Microbiol Infect. 2005 Oct;11(10):834-7. doi: 10.1111/j.1469-0691.2005.01236.x.
2
Loss of the mecA gene during storage of methicillin-resistant Staphylococcus aureus strains.耐甲氧西林金黄色葡萄球菌菌株储存期间mecA基因的缺失
J Clin Microbiol. 2005 Mar;43(3):1361-5. doi: 10.1128/JCM.43.3.1361-1365.2005.
3
Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay.通过实时聚合酶链反应检测法直接从鼻拭子标本中检测耐甲氧西林金黄色葡萄球菌。
J Clin Microbiol. 2004 Dec;42(12):5578-81. doi: 10.1128/JCM.42.12.5578-5581.2004.
4
New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci.用于直接从含有葡萄球菌混合物的标本中快速检测耐甲氧西林金黄色葡萄球菌的新型实时聚合酶链反应检测法。
J Clin Microbiol. 2004 May;42(5):1875-84. doi: 10.1128/JCM.42.5.1875-1884.2004.
5
Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community.社区中新的耐甲氧西林金黄色葡萄球菌克隆的传播。
J Clin Microbiol. 2002 Nov;40(11):4289-94. doi: 10.1128/JCM.40.11.4289-4294.2002.
6
Type 1 capsule genes of Staphylococcus aureus are carried in a staphylococcal cassette chromosome genetic element.金黄色葡萄球菌的1型荚膜基因携带在葡萄球菌盒式染色体遗传元件中。
J Bacteriol. 2002 Jul;184(13):3623-9. doi: 10.1128/JB.184.13.3623-3629.2002.
7
Rapid identification of methicillin-resistant Staphylococcus aureus and simultaneous species confirmation using real-time fluorescence PCR.使用实时荧光PCR快速鉴定耐甲氧西林金黄色葡萄球菌并同时进行菌种确认。
J Clin Microbiol. 2000 Jun;38(6):2429-33. doi: 10.1128/JCM.38.6.2429-2433.2000.
8
Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia.产杀白细胞素的泛耐药金黄色葡萄球菌在原发性皮肤感染和肺炎中的作用。
Clin Infect Dis. 1999 Nov;29(5):1128-32. doi: 10.1086/313461.
Zotero 插件