Damante G, Di Lauro R
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5388-92. doi: 10.1073/pnas.88.12.5388.
The DNA binding specificities of the homeodomains contained in thyroid transcription factor 1 and Antennapedia have been compared. The two homeodomains recognize different DNA sequences, despite the similar amino acid sequences of their recognition helix. Mutations that make the recognition helix of thyroid transcription factor 1 identical to the one contained in Antennapedia have no effects on the binding specificity of thyroid transcription factor 1. The exchange of other segments between these two homeodomains allows the identification of the regions responsible for the observed DNA binding specificities. These results indicate that amino acid residues outside of the recognition helix play an important role in the determination of the DNA binding specificities of these two homeodomains.
已对甲状腺转录因子1和触角足蛋白中所含同源结构域的DNA结合特异性进行了比较。尽管它们识别螺旋的氨基酸序列相似，但这两个同源结构域识别不同的DNA序列。使甲状腺转录因子1的识别螺旋与触角足蛋白中的识别螺旋相同的突变，对甲状腺转录因子1的结合特异性没有影响。在这两个同源结构域之间交换其他片段，可以确定负责观察到的DNA结合特异性的区域。这些结果表明，识别螺旋之外的氨基酸残基在决定这两个同源结构域的DNA结合特异性方面起着重要作用。
