Bertozzi Cara C, Chang Cheng-Yi, Jairaj Sonya, Shan Xiaochuan, Huang Jia, Weber Barbara L, Chu Christina S, Carroll Richard G
Abramson Family Cancer Research Institute, Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19104-6160, USA.
In Vitro Cell Dev Biol Anim. 2006 Mar-Apr;42(3-4):58-62. doi: 10.1290/0512084.1.
To increase the efficiency of stable cell line establishment from primary ovarian cancer specimens, we simultaneously initiated cultures under multiple conditions, varying extracellular matrices and the inclusion of supplements (e.g., serum or serum albumin), while minimizing exposure to xenogeneic antigens (e.g., fetal calf serum). Primary cultures were initiated from 30 specimens; cell lines were established from 10 of these for a success rate of 33%. In some instances, multiple cell lines were established from the same specimen. Five lines were characterized extensively with respect to growth properties, antigen expression, and genomic alterations. Although these lines are all low-passage, marked heterogeneity was observed, even between lines derived from the same specimen. The culture approach outlined herein will facilitate generation of reagents useful for many aspects of ovarian cancer biology.
为提高从原发性卵巢癌标本建立稳定细胞系的效率，我们在多种条件下同时启动培养，改变细胞外基质并添加补充剂（如血清或血清白蛋白），同时尽量减少对外源抗原（如胎牛血清）的暴露。从30个标本开始进行原代培养；从其中10个标本建立了细胞系，成功率为33%。在某些情况下，从同一标本建立了多个细胞系。对5个细胞系的生长特性、抗原表达和基因组改变进行了广泛表征。尽管这些细胞系均为低传代，但即使是来自同一标本的细胞系之间也观察到明显的异质性。本文概述的培养方法将有助于生成对卵巢癌生物学多个方面有用的试剂。
