Rachmiel Marianna, Bloch Olga, Bistritzer Tzvi, Weintrob Naomi, Ofan Regina, Koren-Morag Nira, Rapoport Micha J
Department of Pediatrics, Assaf Harofeh Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
Cytokine. 2006 May;34(3-4):170-6. doi: 10.1016/j.cyto.2006.04.012. Epub 2006 Jun 12.
T1DM and asthma are mediated by opposite arms of the cellular immune system namely T helper (Th)1 and Th2 CD4(+) cells, respectively. Our aim was to characterize the Th1/Th2 cytokine balance in patients with both T1DM and asthma.
Forty-four patients, mean age 19 years were matched by gender and age, to 4 paired groups: T1DM and asthma, asthma only, T1DM only and healthy controls. Peripheral blood mononuclear cells (PBMC) were stimulated in vitro with disease-specific recombinant antigens; glutamic acid decarboxylase and house dust mite (Der p1 antigen) for T1DM and asthma, respectively, and non-specific mitogens; phytohemaglutinin (PHA), tetanus toxin and anti-CD3 mAb. ELISPOT and ELISA technique were used to determine INF-gamma, IL-2, IL-4, IL-13 and IL-10 expression.
Patients with T1DM and asthma demonstrated a similar cytokine pattern but lower Th1/Th2 ratio compared to patients with T1DM only. The Th2 cytokines response to Der p1 was enhanced in patients with both diseases compared to controls. The IL-10 overall secretion was higher in patients with both diseases compared to one disease only.
The Th1 and Th2 secretory pattern of patients with T1DM and asthma combines features of both diseases suggesting a unique Th1/Th2 balance.
1型糖尿病（T1DM）和哮喘分别由细胞免疫系统的相反分支介导，即辅助性T（Th）1和Th2 CD4(+)细胞。我们的目的是描述同时患有T1DM和哮喘的患者的Th1/Th2细胞因子平衡。
44名平均年龄为19岁的患者按性别和年龄匹配，分为4组配对组：T1DM合并哮喘组、单纯哮喘组、单纯T1DM组和健康对照组。外周血单个核细胞（PBMC）在体外分别用疾病特异性重组抗原刺激；T1DM用谷氨酸脱羧酶，哮喘用屋尘螨（Der p1抗原），以及非特异性丝裂原；植物血凝素（PHA）、破伤风毒素和抗CD3单克隆抗体。采用酶联免疫斑点法（ELISPOT）和酶联免疫吸附测定法（ELISA）技术测定INF-γ、IL-2、IL-4、IL-13和IL-10的表达。
与单纯T1DM患者相比，T1DM合并哮喘患者表现出相似的细胞因子模式，但Th1/Th2比值较低。与对照组相比，两种疾病患者对Der p1的Th2细胞因子反应增强。与仅患一种疾病的患者相比，两种疾病患者的IL-10总体分泌更高。
T1DM合并哮喘患者的Th1和Th2分泌模式结合了两种疾病的特征，提示存在独特的Th1/Th2平衡。
