厌氧生物的磷酸酶活性。
Phosphatase activity of anaerobic organisms.
作者信息
Porschen R K, Spaulding E H
出版信息
Appl Microbiol. 1974 Apr;27(4):744-7. doi: 10.1128/am.27.4.744-747.1974.
Abstract
Anaerobic organisms were tested for phosphatase activity in different pH ranges. Several groups of organisms displayed characteristic patterns. Bacteroides fragilis, B. melaninogenicus, and B. ruminicola produced phosphatase with strongest activity at pH 8.6. Fusobacterium mortiferum was the only species of this genus to show strong hydrolysis. The enzyme was active in both acid and alkaline ranges. The activity of gram-positive organisms was variable, the most active groups being Clostridium perfringens, Peptostreptococcus intermedius, P. micros, and Peptococcus constellatus. The incorporation of phosphatase activity into the identification scheme of anaerobes seems feasible. There was a correlation of hydrolysis with several important pathogens.
摘要
对厌氧生物在不同pH范围内的磷酸酶活性进行了测试。几组生物呈现出特征性模式。脆弱拟杆菌、产黑素拟杆菌和瘤胃拟杆菌在pH 8.6时产生的磷酸酶活性最强。致死梭杆菌是该属中唯一显示出强烈水解作用的物种。该酶在酸性和碱性范围内均有活性。革兰氏阳性菌的活性各不相同，活性最强的组为产气荚膜梭菌、中间消化链球菌、微小消化链球菌和星座消化球菌。将磷酸酶活性纳入厌氧菌鉴定方案似乎是可行的。水解作用与几种重要病原体之间存在相关性。
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