Petersen E, Edvinsson B, Lundgren B, Benfield T, Evengård B
Department of Clinical Bacteriology F82, Karolinska Institutet at Karolinska University Hospital-Huddinge, 14186, Stockholm, Sweden.
Eur J Clin Microbiol Infect Dis. 2006 Jun;25(6):401-4. doi: 10.1007/s10096-006-0156-5.
The aim of the study presented here was to evaluate the use of PCR for improving the diagnosis of Toxoplasma gondii infection in immunocompromised hosts. Three hundred thirty-two bronchoalveolar lavage (BAL) fluid samples were analyzed by real-time PCR targeting a 529 bp element of T. gondii. In positive samples, the genotype of the parasite was determined by sequence analysis of the GRA6 gene. Positive results were achieved for 2% (7/332) of the samples tested. Genotyping was possible in two samples and revealed GRA6 type II T. gondii. PCR for detecting T. gondii in BAL samples should be performed in all immunosuppressed HIV-positive patients with symptoms of a systemic infection of unknown etiology. Trimethoprim-sulfamethoxazole prophylaxis does not exclude concomitant infection with T. gondii.
本文所呈现的研究目的是评估聚合酶链反应（PCR）在改善免疫功能低下宿主弓形虫感染诊断方面的应用。通过针对弓形虫529 bp元件的实时PCR分析了332份支气管肺泡灌洗（BAL）液样本。在阳性样本中，通过对GRA6基因进行序列分析来确定寄生虫的基因型。在所检测的样本中，2%（7/332）获得了阳性结果。两个样本能够进行基因分型，结果显示为GRA6 II型弓形虫。对于所有有不明病因全身感染症状的免疫抑制HIV阳性患者，均应进行PCR检测BAL样本中的弓形虫。甲氧苄啶 - 磺胺甲恶唑预防并不能排除同时感染弓形虫。
