Roth A, Roth B, Höffken G, Steuber S, Khalifa K I, Janitschke K
Department of Clinical Parasitology, Robert Koch Institute, Berlin, Germany.
Eur J Clin Microbiol Infect Dis. 1992 Dec;11(12):1177-81. doi: 10.1007/BF01961141.
Bronchoalveolar lavage (BAL) fluid from 47 immunocompromised patients (26 with AIDS and 21 patients on immunosuppressive therapy) was analysed for the presence of Toxoplasma gondii DNA by means of the polymerase chain reaction (PCR). Specific target DNA derived from the B1 and P30 gene of Toxoplasma gondii was detected in BAL fluids from three patients with AIDS (6.4%). Pneumonia as the presenting feature of disseminated toxoplasmosis was confirmed by both clinical findings and by detection of Toxoplasma gondii DNA in blood obtained from two patients. The findings indicate that PCR has potential value in the detection of Toxoplasma gondii as an etiologic agent of atypical pneumonia in immunocompromised patients.
通过聚合酶链反应（PCR）分析了47例免疫功能低下患者（26例艾滋病患者和21例接受免疫抑制治疗的患者）的支气管肺泡灌洗（BAL）液中是否存在刚地弓形虫DNA。在3例艾滋病患者（6.4%）的BAL液中检测到源自刚地弓形虫B1和P30基因的特异性靶DNA。两名患者血液中刚地弓形虫DNA的检测以及临床发现均证实，肺炎是播散性弓形虫病的首发症状。这些发现表明，PCR在检测免疫功能低下患者非典型肺炎的病原体刚地弓形虫方面具有潜在价值。
