采用支气管肺泡灌洗液进行定量竞争性聚合酶链反应诊断艾滋病患者弓形虫病
Quantitative competitive PCR with bronchoalveolar lavage fluid for diagnosis of toxoplasmosis in AIDS patients.
作者信息
Bretagne S, Costa J M, Fleury-Feith J, Poron F, Dubreuil-Lemaire M L, Vidaud M
机构信息
Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor, Créteil, France.
出版信息
J Clin Microbiol. 1995 Jun;33(6):1662-4. doi: 10.1128/jcm.33.6.1662-1664.1995.
Abstract
Of 121 AIDS patients, 12 (10%) had Toxoplasma gondii DNA detected by competitive PCR in their bronchoalveolar lavage samples. Quantitation of the PCR results showed a correlation between the parasite burden and the serum lactic dehydrogenase titer. Quantitative competitive PCR could be useful to assess the diagnosis and the severity of pulmonary toxoplasmosis.
摘要
在121例艾滋病患者中，12例（10%）的支气管肺泡灌洗样本经竞争性聚合酶链反应检测出弓形虫DNA。聚合酶链反应结果的定量分析显示，寄生虫负荷与血清乳酸脱氢酶滴度之间存在相关性。定量竞争性聚合酶链反应可能有助于评估肺弓形虫病的诊断和严重程度。
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