Lavrard I, Chouaid C, Roux P, Poirot J L, Marteau M, Lemarchand B, Meyohas M C, Olivier J L
Département de Biologie Moléculaire, Hôpital Saint-Antoine, Paris, France.
Eur Respir J. 1995 May;8(5):697-700.
Toxoplasmosis is a serious opportunistic infection in patients with acquired immunodeficiency syndrome (AIDS). The lung is a major site of infection after the central nervous system. The aim of the study was to assess the polymerase chain reaction (PCR) and cell culture for the detection of Toxoplasma gondii. One hundred and thirty two human immunodeficiency virus (HIV)-infected patients with respiratory manifestations, who underwent fibreoptic bronchoalveolar lavage, were investigated. Detection of Toxoplasma gondii was compared using three techniques: Giemsa staining; polymerase chain reaction with specific primers derived from the P30 gene; and culture on the MRC5 cell line. Toxoplasma gondii was detected in the same four samples by all three techniques. We conclude that PCR adds little to conventional (and cheaper) tools already used to diagnose pulmonary toxoplasmosis.
弓形虫病是获得性免疫缺陷综合征（艾滋病）患者中一种严重的机会性感染。肺部是继中枢神经系统之后的主要感染部位。本研究的目的是评估聚合酶链反应（PCR）和细胞培养用于检测刚地弓形虫的效果。对132例有呼吸道表现且接受了纤维支气管镜肺泡灌洗的人类免疫缺陷病毒（HIV）感染患者进行了调查。使用三种技术比较刚地弓形虫的检测情况：吉姆萨染色；使用源自P30基因的特异性引物进行聚合酶链反应；以及在MRC5细胞系上培养。所有三种技术在相同的4个样本中均检测到了刚地弓形虫。我们得出结论，PCR对已用于诊断肺部弓形虫病的传统（且更便宜）工具帮助不大。
