Bachmann P A, ter Meulen V, Jentsch G, Appel M, Iwasaki Y, Meyermann R, Koprowski H, Mayr A
Arch Virol. 1975;48(2):107-20. doi: 10.1007/BF01318144.
Isolation of a viral agent (107) directly from brain explants of a 15-month-old heifer with symptoms of a sporadic encephalomyelitis is described. The virus shares properties with the paramyxovirus family. It grows in a variety of cell cultures from different species, and induces nuclear and cytoplasmic inclusion bodies in infected cells. Nucleocapsids measuring 17 nm in diameter were found in the nucleus and cytoplasm of these cells when studied electron microscopically, thus indicating a close relationship of the agent to the measles-distemper-rinderpest group. No infectious virus was released from infected cells, although alignment of nucleocapsids was observed beneath the cell membrane, and no hemagglutinating activity could be detected with the methods employed. The 107 agent was compared serologically with parainfluenza viruses type 1, 2 and 3, simian virus 5, mumps and Newcastle disease virus (NDV), two bovine respiratory syncytial viruses and measles/subacute sclerosing panencephalitis, distemper and rinderpest viruses, always using 107 virus infected CV1 cells and antiserum of the different viruses in indirect FA tests. Positive FA reactions were observed only with two sera obtained from SSPE patients with high antibody titer to SSPE virus, and with one rabbit-anti-rinderpest serum. The titers of these sera to 107 virus, however, were significantly lower than those against homologous viruses. Five out of 9 sera from randomly selected healthy cattle showed antibody titers between 1:10 and 1:80 to 107 virus in FA tests. The significance of these results is discussed with respect to the epidemiology of SSPE in children and its possible implication with rinderpest in Europe.
本文描述了从一头患有散发性脑脊髓炎症状的15月龄小母牛的脑外植体中直接分离出一种病毒制剂（107）。该病毒具有副粘病毒科的特性。它能在来自不同物种的多种细胞培养物中生长，并在受感染细胞中诱导核内和胞质内包涵体形成。在电子显微镜下研究时，在这些细胞的细胞核和细胞质中发现了直径为17nm的核衣壳，这表明该制剂与麻疹 - 犬瘟热 - 牛瘟病毒组密切相关。尽管在细胞膜下观察到核衣壳排列，但未从受感染细胞中释放出传染性病毒，并且采用所使用的方法未检测到血凝活性。在间接荧光抗体试验中，始终使用107病毒感染的CV1细胞和不同病毒的抗血清，将107制剂与1型、2型和3型副流感病毒、猴病毒5、腮腺炎病毒和新城疫病毒（NDV）、两种牛呼吸道合胞病毒以及麻疹/亚急性硬化性全脑炎、犬瘟热和牛瘟病毒进行血清学比较。仅在从患有高滴度抗SSPE病毒抗体的SSPE患者获得的两份血清以及一份兔抗牛瘟血清中观察到阳性荧光抗体反应。然而，这些血清对107病毒的滴度明显低于对同源病毒的滴度。在随机选择的9头健康牛的血清中，有5份在荧光抗体试验中对107病毒的抗体滴度在1:10至1:80之间。针对儿童SSPE的流行病学及其在欧洲与牛瘟的可能关联，对这些结果的意义进行了讨论。
