Watkins J F, Sanger C
Br J Cancer. 1977 Jun;35(6):785-94. doi: 10.1038/bjc.1977.120.
A new, highly differentiated line of cells derived from adenocarcinoma of the rectum (HT55) is described. This line is noteworthy for the following features: 1. The role played in its development by the use of UV-inactivated Sendai virus to attach tumour cell clumps to plastic bottles. 2. Evidence that it produces RNA-containing material of density 1-5--1-16 g/ml. 3. Induction of bone formation in the stroma when grown in athymic mice. 4. Stimulation of primary CBA mouse embryo fibroblasts to form a transient nodule when mixed with them and injected into adult CBA mice. The karyotype and growth-cycle characteristics of the line are described.
本文描述了一种从直肠腺癌（HT55）中衍生出的新型、高度分化的细胞系。该细胞系具有以下值得注意的特征：1. 在其培养过程中，使用紫外线灭活的仙台病毒将肿瘤细胞团附着于塑料瓶所起的作用。2. 有证据表明它能产生密度为1.5 - 1.16 g/ml的含RNA物质。3. 在无胸腺小鼠体内生长时可诱导基质中骨形成。4. 与原代CBA小鼠胚胎成纤维细胞混合并注射到成年CBA小鼠体内时，能刺激其形成短暂结节。文中还描述了该细胞系的核型和生长周期特征。
