Suhagia Bhanubhai N, Shah Shailesh A, Rathod Ishwarsinh S, Patel Haresh M, Shah Dinesh R, Marolia Bhavin P
Department of Quality Assurance, L. M. College of Pharmacy.
Anal Sci. 2006 May;22(5):743-5. doi: 10.2116/analsci.22.743.
A simple and sensitive high-performance thin-layer chromatography (HPTLC) method has been developed for the quantitative estimation of gatifloxacin and ornidazole in its combined dosage forms. Gatifloxacin and ornidazole were chromatographed on silica Gel 60 F(254) TLC plate using n-butanol:methanol:ammonia (6 M) (8:1:1.5 v/v) as the mobile phase and scanned at 302 nm using a Camag TLC Scanner 3. The R(f) value of gatifloxacin and ornidazole was found to be 0.21 +/- 0.02 and 0.76 +/- 0.04, respectively. The linearity of gatifloxacin and ornidazole were in the range of 100 - 500 ng/spot and 250 - 1250 ng/spot, respectively. The limit of detection was found to be 40 ng/spot for gatifloxacin and 100 ng/spot for ornidazole. The proposed method was applied for the determination of gatifloxacin and ornidazole in combined dosage forms.
已开发出一种简单灵敏的高效薄层色谱（HPTLC）方法，用于定量测定加替沙星和奥硝唑复方制剂中的含量。加替沙星和奥硝唑在硅胶60 F(254) TLC板上进行色谱分析，以正丁醇：甲醇：氨水（6 M）（8:1:1.5 v/v）为流动相，使用Camag TLC Scanner 3在302 nm波长处进行扫描。加替沙星和奥硝唑的比移值（R(f)）分别为0.21±0.02和0.76±0.04。加替沙星和奥硝唑的线性范围分别为100 - 500 ng/斑点和250 - 1250 ng/斑点。加替沙星的检测限为40 ng/斑点，奥硝唑的检测限为100 ng/斑点。该方法用于测定加替沙星和奥硝唑复方制剂中的含量。
