Department of Chemistry, Kyungpook National University, Daegu 702-701, South Korea.
J Fluoresc. 2011 Jul;21(4):1539-45. doi: 10.1007/s10895-011-0842-2. Epub 2011 Jan 19.
A sensitive and rapid flow-injection chemiluminescence (CL) method has been developed for the determination of gatifloxacin in pharmaceutical preparations and biological samples. The method is based on the enhancing effect of gatifloxacin on CL emission generated by the interaction of Ce (IV) in sulphuric acid and sodium hyposulphite (Na(2)S(2)O(4)) sensitized by sodium dodecyl benzene sulfonate (SDBS). Strong CL emission was observed when gatifloxacin was injected into the Ce (IV) in sulphuric acid and Na(2)S(2)O(4) solution incorporated with SDBS in a flow-cell. Several experimental parameters affecting the CL reaction were investigated and optimized systematically. Under the optimum conditions, it was found that the CL intensity is proportional to the concentration of gatifloxacin in the range of 1.12 × 10(-11)-4.40 × 10(-9) g mL(-1) with a co-relation coefficient of 0.9994. The limit of detection was found to be 4.87 × 10(-12) g mL(-1) and the relative standard deviation (RSD, n=7) was 1.8% for 4 × 10(-8) g mL(-1) of GFLX. The proposed method offers higher sensitivity, wide linear range and better stability without requiring sophisticated instrumentation. Thus, the proposed method has been successfully applied to the determination of gatifloxacin in pharmaceuticals, serum and human urine.
一种灵敏、快速的流动注射化学发光（CL）法已被开发出来，用于测定药物制剂和生物样品中的加替沙星。该方法基于加替沙星对硫酸中 Ce（IV）与低亚硫酸钠（Na 2 S 2 O 4 ）相互作用产生的 CL 发射的增强效应，该效应被十二烷基苯磺酸钠（SDBS）敏化。当加替沙星注入包含 SDBS 的硫酸和 Na 2 S 2 O 4 溶液中的流动池时，会观察到强烈的 CL 发射。系统地研究和优化了影响 CL 反应的几个实验参数。在最佳条件下，发现 CL 强度与加替沙星的浓度在 1.12×10（-11）-4.40×10（-9）g mL（-1）范围内成正比，相关系数为 0.9994。检测限为 4.87×10（-12）g mL（-1），对于 4×10（-8）g mL（-1）的 GFLX，相对标准偏差（RSD，n=7）为 1.8%。该方法具有较高的灵敏度、较宽的线性范围和较好的稳定性，无需复杂的仪器。因此，该方法已成功应用于药物、血清和人尿中加替沙星的测定。
