磷脂酰肌醇周转增强与丝裂原激活淋巴细胞之间的关系。
Relationship between enhanced turnover of phosphatidylinositol and lymphocyte activation by mitogens.
作者信息
Maino V C, Hayman M J, Crumpton M J
出版信息
Biochem J. 1975 Jan;146(1):247-52. doi: 10.1042/bj1460247.
Abstract
- Various lectins [phaseolus vulgaris phytohaemagglutinin, Glycine max (soy-bean) agglutinin, Triticum vulgaris (wheat-germ) agglutinin and Axinella polyploides agglutinin] and antibodies to pig Ig (immunoglobulin) that are found by pig lymphocytes were assessed in terms of their capacities to stimulate lymphocyte transformation and to enhance phosphatidylinositol turnover. Transformation was measured after 45h of culture by incorporation of [6-(3)H]thymidine into DNA, whereas phosphatidylinositol metabolism was assessed after 1h of cultuis and G. max agglutinins and rabbit antibodies to pig Ig) increased phosphatidylinositol turnover, but non-transforming agents (T. vulgaris and A. polyploides agglutinins and Fab fragments of rabbit antibodies to pig Ig) failed to induce any significant enhancement. Subsequent cross-linkage of the bound, non-transforming Fab fragments with a goat antiserum to rabbit Ig stimulated transformation and phosphatidylinositol turnover. 3. Each transforming agent gave characteristic optimal dose responses that were similar for both phosphatidylinositol turnover and transformation. 4. The results indicate that activation of T- and B-lymphocytes is accompanied by enhanced phosphatidylinositol turnover and that in the case of B-cells this enhancement depends on the cross-linkage of surface receptors. They are consistent with the proposal that turnover represents an essential early step in the transformation process.
摘要
- 对猪淋巴细胞发现的多种凝集素（菜豆植物血凝素、大豆凝集素、小麦胚芽凝集素和多倍体轴海绵凝集素）以及抗猪Ig（免疫球蛋白）抗体，就其刺激淋巴细胞转化和增强磷脂酰肌醇代谢的能力进行了评估。培养45小时后，通过将[6-(3)H]胸腺嘧啶核苷掺入DNA来测定转化情况，而磷脂酰肌醇代谢在培养1小时后进行评估。大豆凝集素和兔抗猪Ig抗体增加了磷脂酰肌醇代谢，但非转化剂（小麦胚芽凝集素和多倍体轴海绵凝集素以及兔抗猪Ig抗体的Fab片段）未能诱导任何显著增强。随后，将结合的非转化Fab片段与山羊抗兔Ig抗血清进行交联，刺激了转化和磷脂酰肌醇代谢。3. 每种转化剂都给出了特征性的最佳剂量反应，磷脂酰肌醇代谢和转化的反应相似。4. 结果表明，T淋巴细胞和B淋巴细胞的激活伴随着磷脂酰肌醇代谢增强，并且就B细胞而言，这种增强依赖于表面受体的交联。它们与磷脂酰肌醇代谢是转化过程中一个重要早期步骤的提议一致。
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