Caproni Marzia, Torchia Daniele, Antiga Emiliano, Volpi Walter, del Bianco Elena, Fabbri Paolo
Department of Dermatological Sciences, University of Florence, Florence, Italy.
J Clin Immunol. 2006 Jul;26(4):370-5. doi: 10.1007/s10875-006-9034-2. Epub 2006 Jun 14.
Only very recently studies were conducted in order to evaluate the impact of regulatory T (Treg) cells in the pathophysiology of atopic dermatitis (AD). Nine adult patients with moderate-to-severe AD in riacutization period of a chronic disease were given tacrolimus ointment, while seven hydrocortisone butyrate ointment, that served as controls. We performed lesional-skin biopsies before and after treatment, that were stained immunohistochemically with monoclonal antibodies to CD4, CD25, forkhead/winged helix transcription factor (FoxP3), interleukin (IL)-10 and transforming growth factor (TGF)-beta. CD4+ cells were significantly reduced in post-treatment series. Tacrolimus treatment achieved a significant reduction of CD25+ cells. FoxP3+ cells were present in untreated AD lesions. Both treatments did not significantly modify the number of FoxP3+ cells. The number of IL-10+ cells increased in post-treatment series. Tacrolimus enhanced the production of TGF-beta, while hydrocortisone did not. Restoration of TGF-beta-producing Treg cells may represent another important pharmacodynamic effect of tacrolimus on AD.
直到最近才开展了一些研究，以评估调节性T（Treg）细胞在特应性皮炎（AD）病理生理学中的作用。9例处于慢性病急性发作期的中重度AD成年患者外用他克莫司软膏，7例外用丁酸氢化可的松软膏作为对照。我们在治疗前后进行皮损活检，并用抗CD4、CD25、叉头/翼状螺旋转录因子（FoxP3）、白细胞介素（IL）-10和转化生长因子（TGF）-β的单克隆抗体进行免疫组织化学染色。治疗后CD4+细胞显著减少。他克莫司治疗使CD25+细胞显著减少。未经治疗的AD皮损中存在FoxP3+细胞。两种治疗均未显著改变FoxP3+细胞数量。治疗后IL-10+细胞数量增加。他克莫司可增强TGF-β的产生，而氢化可的松则不能。恢复产生TGF-β的Treg细胞可能是他克莫司对AD的另一个重要药效学作用。
