Péronnet F, Massicotte D, Folch N, Melin B, Koulmann N, Jimenez C, Bourdon L, Launay J-C, Savourey G
Département de kinésiologie, Université de Montréal, H3C 3J7, Montreal, QC, Canada.
Eur J Appl Physiol. 2006 Jul;97(5):527-34. doi: 10.1007/s00421-006-0164-2. Epub 2006 May 23.
Energy substrate oxidation was measured using indirect respiratory calorimetry combined with tracer technique in five healthy young male subjects, during a 80-min exercise period on ergocycle with ingestion of 140 g of (13)C-labelled glucose, in normoxia and acute hypobaric hypoxia (445 mmHg or 4,300 m), at the same relative [77% V(.-)((O)(2)(max))] and absolute workload (161+/-8 W, corresponding to 77 and 54% V(.-)((O)(2)(max)) in hypoxia and normoxia). The oxidation rate of exogenous glucose was not significantly different in the three experimental situations: 21.4+/-2.9, 20.2+/-1.2 and 17.2+/-0.6 g over the last 40 min of exercise at approximately 77 and approximately 54% V(.-)((O)(2)(max)) in normoxia and in hypoxia, respectively, providing 12.5+/-1.5, 16.8+/-1.1 and 14.9+/-1.1% of the energy yield, although ingestion of glucose during exercise resulted in a higher plasma glucose concentration in hypoxia than normoxia. The contribution of carbohydrate (CHO) oxidation to the energy yield was significantly higher in hypoxia (92.0+/-2.1%) than in normoxia for both a given absolute (75.3+/-5.2%) and relative workload (78.1+/-1.8%). This greater reliance on CHO oxidation in hypoxia was entirely due to the significantly larger contribution of endogenous glucose oxidation to the energy yield: 75.9+/-1.7% versus 66.6+/-3.3 and 55.2+/-3.7% in normoxia at the same relative and absolute workload.
在常氧和急性低压缺氧（445 mmHg或海拔4300米）条件下，对5名健康年轻男性受试者进行了80分钟的功率自行车运动，并摄入140克（13）C标记的葡萄糖，期间使用间接呼吸热量测定法结合示踪技术测量能量底物氧化。在相同的相对[77% V̇O₂max]和绝对工作量（161±8 W，分别对应缺氧和常氧时V̇O₂max的77%和54%）下进行测量。在三种实验情况下，外源性葡萄糖的氧化速率没有显著差异：在常氧和缺氧条件下，运动最后40分钟，分别约为77%和54% V̇O₂max时，外源性葡萄糖氧化速率分别为21.4±2.9、20.2±1.2和17.2±0.6克，分别提供能量产出的12.5±1.5%、16.8±1.1%和14.9±1.1%，尽管运动期间摄入葡萄糖导致缺氧时血浆葡萄糖浓度高于常氧。对于给定的绝对工作量（75.3±5.2%）和相对工作量（78.1±1.8%），碳水化合物（CHO）氧化对能量产出的贡献在缺氧（92.0±2.1%）时显著高于常氧。在缺氧条件下对CHO氧化的更大依赖完全是由于内源性葡萄糖氧化对能量产出的贡献显著更大：在相同的相对和绝对工作量下，常氧时分别为66.6±3.3%和55.2±3.7%，而缺氧时为75.9±1.7%。
