Shiomi Kazu, Miyamoto Hideaki, Segawa Tatsuya, Hagiwara Yoshiaki, Ota Akinobu, Maeda Masahiro, Takahashi Kazuhisa, Masuda Kimihiko, Sakao Yukinori, Hino Okio
Department of General Thoracic Surgery, Respiratory Medicine, Juntendo University, School of Medicine, 2-1-1 Hongo, Tokyo 113-8421, Japan.
Cancer Sci. 2006 Sep;97(9):928-32. doi: 10.1111/j.1349-7006.2006.00246.x. Epub 2006 Jun 7.
We have developed a novel enzyme-linked immunosorbent assay (ELISA) system for the detection of N-ERC/mesothelin in the serum of mesothelioma patients and have begun to examine its clinical usefulness. N-ERC/mesothelin is a 31-kDa protein that forms the N-terminal fragment of the full-length 71-kDa ERC/mesothelin protein, and is physiologically secreted into the blood of mesothelioma patients where it can be detected using our sandwich ELISA containing two antibodies (rabbit polyclonal anti-ERC/mesothelin antibody-282 and mouse monoclonal antibody 7E7). Our ELISA system has thus far detected much higher serum levels of N-ERC/mesothelin in mesothelioma patients than in healthy controls or patients with other lung or pleural diseases. In conclusion, N-ERC/mesothelin is a promising candidate tumor marker for mesothelioma.
我们开发了一种新型酶联免疫吸附测定（ELISA）系统，用于检测间皮瘤患者血清中的N-ERC/间皮素，并已开始研究其临床实用性。N-ERC/间皮素是一种31 kDa的蛋白质，它构成全长71 kDa的ERC/间皮素蛋白的N端片段，在生理状态下会分泌到间皮瘤患者的血液中，使用我们包含两种抗体（兔多克隆抗ERC/间皮素抗体-282和小鼠单克隆抗体7E7）的夹心ELISA可以检测到它。到目前为止，我们的ELISA系统检测到间皮瘤患者血清中的N-ERC/间皮素水平比健康对照者或患有其他肺部或胸膜疾病的患者高得多。总之，N-ERC/间皮素是一种有前景的间皮瘤候选肿瘤标志物。
