• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

色氨酸tRNA与禽成髓细胞瘤病毒35S RNA杂交及在体外引发逆转录的能力。

Ability of tryptophan tRNA to hybridize with 35S RNA of avian myeloblastosis virus and to prime reverse transcription in vitro.

作者信息

Waters L C, Mullin B C, Ho T, Yang W K

出版信息

Proc Natl Acad Sci U S A. 1975 Jun;72(6):2155-9. doi: 10.1073/pnas.72.6.2155.

DOI:10.1073/pnas.72.6.2155
PMID:49054
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC432715/
Abstract

Selected species of 4S RNA of chick embryo cells will hybridize in vitro with 35S RNA of avian myeloblastosis virus. A major tRNA component of the hybridizable 4S RNA is tryptophan tRNA. A hybrid prepared from purified tryptophan tRAN and 35S RNA of avian myeloblastosis virus in vitro is an efficient templateprimer for DNA synthesis catalyzed by reverse transcriptase (RNA-dependent DNA polymerase).

摘要

鸡胚细胞4S RNA的某些选定种类能在体外与禽成髓细胞瘤病毒的35S RNA杂交。可杂交的4S RNA的一种主要tRNA成分是色氨酸tRNA。由纯化的色氨酸tRNA和禽成髓细胞瘤病毒的35S RNA在体外制备的杂交体是逆转录酶（RNA依赖性DNA聚合酶）催化DNA合成的有效模板引物。

相似文献

1
Ability of tryptophan tRNA to hybridize with 35S RNA of avian myeloblastosis virus and to prime reverse transcription in vitro.色氨酸tRNA与禽成髓细胞瘤病毒35S RNA杂交及在体外引发逆转录的能力。
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2155-9. doi: 10.1073/pnas.72.6.2155.
2
tRNATrp (bovine) binding to the reverse transcriptase of avian myeloblastosis virus and function as a heterologous primer.牛源色氨酸转运RNA（tRNATrp）与禽成髓细胞瘤病毒逆转录酶结合并作为异源引物发挥作用。
Proc Natl Acad Sci U S A. 1977 May;74(5):1889-93. doi: 10.1073/pnas.74.5.1889.
3
Primer recognition by avian myeloblastosis virus RNA-directed DNA polymerase.禽成髓细胞瘤病毒RNA指导的DNA聚合酶对引物的识别
J Virol. 1977 Mar;21(3):1236-9. doi: 10.1128/JVI.21.3.1236-1239.1977.
4
Specific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase).色氨酸转移RNA与禽成髓细胞瘤病毒RNA依赖性DNA聚合酶（逆转录酶）的特异性结合。
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2535-9. doi: 10.1073/pnas.72.7.2535.
5
Interaction of tryptophan tRNA and avian myeloblastosis virus reverse transcriptase: further characterization of the binding reaction.色氨酸转运RNA与禽成髓细胞瘤病毒逆转录酶的相互作用：结合反应的进一步表征
Biochemistry. 1977 Aug 9;16(16):3625-32. doi: 10.1021/bi00635a019.
6
Primer tRNATrp enhances the inhibition of avian myeloblastosis virus reverse transcriptase by pyridoxal-5'-phosphate.引物tRNATrp增强了磷酸吡哆醛对禽成髓细胞瘤病毒逆转录酶的抑制作用。
Biochem Biophys Res Commun. 1979 May 14;88(1):9-15. doi: 10.1016/0006-291x(79)91689-9.
7
Structural features required for the binding of tRNATrp to avian myeloblastosis virus reverse transcriptase.tRNATrp与禽成髓细胞瘤病毒逆转录酶结合所需的结构特征。
Nucleic Acids Res. 1983 Jul 25;11(14):4823-33. doi: 10.1093/nar/11.14.4823.
8
tRNA's associated with the 70S RNA of avian myeloblastosis virus.与禽成髓细胞瘤病毒70S RNA相关的转运RNA
J Virol. 1975 Dec;16(6):1608-14. doi: 10.1128/JVI.16.6.1608-1614.1975.
9
RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus.禽成髓细胞瘤病毒逆转录酶用于合成反互补DNA时所使用的RNA引物。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1316-20. doi: 10.1073/pnas.77.3.1316.
10
Study of the interactions between avian myeloblastosis virus reverse transcriptase and primer tRNA. Affinity labeling and inactivation of the enzyme by periodate-treated tRNATrp.禽成髓细胞瘤病毒逆转录酶与引物tRNA相互作用的研究。经高碘酸盐处理的tRNATrp对该酶的亲和标记及失活作用。
Nucleic Acids Res. 1980 Sep 11;8(17):4009-20. doi: 10.1093/nar/8.17.4009.

引用本文的文献

1
Mechanistic differences between nucleic acid chaperone activities of the Gag proteins of Rous sarcoma virus and human immunodeficiency virus type 1 are attributed to the MA domain. Rous 肉瘤病毒和人类免疫缺陷病毒 1 的 Gag 蛋白的核酸伴侣活性的机制差异归因于 MA 结构域。
J Virol. 2014 Jul;88(14):7852-61. doi: 10.1128/JVI.00736-14. Epub 2014 Apr 30.
2
Inability of human immunodeficiency virus type 1 produced in murine cells to selectively incorporate primer formula.在鼠细胞中产生的1型人类免疫缺陷病毒无法选择性地掺入引物配方。
J Virol. 2008 Dec;82(24):12049-59. doi: 10.1128/JVI.01744-08. Epub 2008 Oct 8.
3
Defective replication in human immunodeficiency virus type 1 when non-primers are used for reverse transcription.当使用非引物进行逆转录时，1型人类免疫缺陷病毒的复制缺陷。
J Virol. 2005 Jul;79(14):9081-7. doi: 10.1128/JVI.79.14.9081-9087.2005.
4
Effect of altering the tRNA(Lys)(3) concentration in human immunodeficiency virus type 1 upon its annealing to viral RNA, GagPol incorporation, and viral infectivity.改变人免疫缺陷病毒1型中tRNA(Lys)(3)浓度对其与病毒RNA退火、GagPol掺入及病毒感染性的影响。
J Virol. 2002 Sep;76(18):9096-102. doi: 10.1128/jvi.76.18.9096-9102.2002.
5
Primer tRNAs for reverse transcription.用于逆转录的引物tRNA。
J Virol. 1997 Nov;71(11):8087-95. doi: 10.1128/JVI.71.11.8087-8095.1997.
6
Identification of tRNAs incorporated into wild-type and mutant human immunodeficiency virus type 1.整合到野生型和突变型人类免疫缺陷病毒1型中的转运RNA的鉴定
J Virol. 1993 Jun;67(6):3246-53. doi: 10.1128/JVI.67.6.3246-3253.1993.
7
3' truncated tRNAArg is essential for in vitro specific cleavage of partially synthesized mouse 18S rRNA.3' 截短的tRNAArg对于体外特异性切割部分合成的小鼠18S rRNA至关重要。
Nucleic Acids Res. 1993 Oct 11;21(20):4696-702. doi: 10.1093/nar/21.20.4696.
8
Incorporation of excess wild-type and mutant tRNA(3Lys) into human immunodeficiency virus type 1.过量野生型和突变型tRNA(3Lys)掺入1型人类免疫缺陷病毒。
J Virol. 1994 Dec;68(12):7676-83. doi: 10.1128/JVI.68.12.7676-7683.1994.
9
Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles.Pr160gag-pol在介导tRNA（Lys）选择性掺入1型人类免疫缺陷病毒颗粒中的作用。
J Virol. 1994 Apr;68(4):2065-72. doi: 10.1128/JVI.68.4.2065-2072.1994.
10
Structural features required for the binding of tRNATrp to avian myeloblastosis virus reverse transcriptase.tRNATrp与禽成髓细胞瘤病毒逆转录酶结合所需的结构特征。
Nucleic Acids Res. 1983 Jul 25;11(14):4823-33. doi: 10.1093/nar/11.14.4823.

本文引用的文献

1
Measurement of 32P activity in a liquid scintillation counter without the use of scintillator.在不使用闪烁体的情况下，通过液体闪烁计数器测量³²P 活度。
Anal Biochem. 1968 Jan;22(1):70-3. doi: 10.1016/0003-2697(68)90260-1.
2
Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.在聚氯三氟乙烯支持的反相色谱柱上改进转运RNA的分离。
Biochim Biophys Acta. 1971 Feb 11;228(3):770-4. doi: 10.1016/0005-2787(71)90748-9.
3
Multiple isoaccepting transfer RNA's in a muouse plasma cell tumor.小鼠浆细胞瘤中的多种同功受体转运RNA
Proc Natl Acad Sci U S A. 1968 Jan;59(1):208-15. doi: 10.1073/pnas.59.1.208.
4
In vitro association of unique species of cellular 4S RNA with 35S RNA of RNA tumor viruses.细胞4S RNA的独特种类与RNA肿瘤病毒35S RNA的体外结合
Biochem Biophys Res Commun. 1974 Sep 23;60(2):489-97. doi: 10.1016/0006-291x(74)90267-8.
5
Virion-associated RNA primer for Rous sarcoma virus DNA synthesis: isolation from uninfected cells.劳氏肉瘤病毒DNA合成的病毒体相关RNA引物：从未感染细胞中分离得到
J Virol. 1974 Jun;13(6):1302-11. doi: 10.1128/JVI.13.6.1302-1311.1974.
6
Methionine transfer RNAs associated with avian oncornavirus 70S RNA.与禽肿瘤病毒70S RNA相关的甲硫氨酸转运RNA
Nature. 1974 Feb 15;247(5441):435-8. doi: 10.1038/247435a0.
7
RNA-directed DNA polymerase of Rous sarcoma virus: initiation of synthesis with 70 S viral RNA as template.劳氏肉瘤病毒的RNA指导DNA聚合酶：以70S病毒RNA为模板起始合成
J Mol Biol. 1973 Sep 5;79(1):163-83. doi: 10.1016/0022-2836(73)90277-5.
8
Amino-acid acceptor activity of the "70S-associated" 4S RNA from avian myeloblastosis virus.来自禽成髓细胞瘤病毒的“70S相关”4S RNA的氨基酸受体活性。
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1184-5. doi: 10.1073/pnas.70.4.1184.
9
Role of subunits of 60 to 70S avian tumor virus ribonucleic acid in its template activity for the viral deoxyribonucleic acid polymerase.60至70S禽肿瘤病毒核糖核酸亚基在其作为病毒脱氧核糖核酸聚合酶模板活性中的作用。
J Virol. 1972 Jul;10(1):23-31. doi: 10.1128/JVI.10.1.23-31.1972.
10
Covalently linked RNA-DNA molecule as initial product of RNA tumour virus DNA polymerase.共价连接的RNA-DNA分子作为RNA肿瘤病毒DNA聚合酶的初始产物。
Nat New Biol. 1971 Sep 29;233(39):131-4. doi: 10.1038/newbio233131a0.