Waters L C, Mullin B C, Ho T, Yang W K
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2155-9. doi: 10.1073/pnas.72.6.2155.
Selected species of 4S RNA of chick embryo cells will hybridize in vitro with 35S RNA of avian myeloblastosis virus. A major tRNA component of the hybridizable 4S RNA is tryptophan tRNA. A hybrid prepared from purified tryptophan tRAN and 35S RNA of avian myeloblastosis virus in vitro is an efficient templateprimer for DNA synthesis catalyzed by reverse transcriptase (RNA-dependent DNA polymerase).
鸡胚细胞4S RNA的某些选定种类能在体外与禽成髓细胞瘤病毒的35S RNA杂交。可杂交的4S RNA的一种主要tRNA成分是色氨酸tRNA。由纯化的色氨酸tRNA和禽成髓细胞瘤病毒的35S RNA在体外制备的杂交体是逆转录酶(RNA依赖性DNA聚合酶)催化DNA合成的有效模板引物。