Service de Biochimie, C.E.N.-Saclay, B.P. no.2, 91190 Gif Sur Yvette, France.
J Virol. 1973 Feb;11(2):238-42. doi: 10.1128/JVI.11.2.238-242.1973.
Turnip yellow mosaic virus (TYMV) RNA treated with snake venom phosphodiesterase accepts cytidine 5'-monophosphate and adenosine 5'-monophosphate (AMP) when it is incubated in the presence of cytidine 5'-triphosphate (CTP), adenosine 5'-triphosphate, and Escherichia coli transfer RNA nucleotidyltransferase; untreated TYMV RNA accepts only AMP. When alpha (32)PCTP was used for terminal labeling, the nearest neighbor analyses and the anallyses after action of various nucleases showed that the sequence of five nucleotides at the 3' end of TYMV RNA is: pGpCpApCpC. A nuclease present in commerical preparations of snake venom phosphodiesterase leads to the fragmentation of TYMV RNA, the 3' end of which is found in a fragment having a sedimentation constant close to 5s.
芜菁黄花叶病毒(TYMV) RNA 经蛇毒磷酸二酯酶处理后，在胞苷 5'-三磷酸(CTP)、腺苷 5'-三磷酸(ATP)和大肠杆菌转移 RNA 核苷酸转移酶存在的条件下孵育时，可接受胞苷 5'-单磷酸(CMP)和腺苷 5'-单磷酸(AMP);未经处理的 TYMV RNA 仅接受 AMP。当使用α(32)PCTP 进行末端标记时，通过对近邻分析和各种核酸酶作用后的分析表明，TYMV RNA 3'末端的五个核苷酸序列为：pGpCpApCpC。商业蛇毒磷酸二酯酶制剂中存在的一种核酸酶导致 TYMV RNA 断裂，其 3'末端存在于沉降常数接近 5s 的片段中。
