Fang Zhi-yuan, Lin Rong, Yuan Bing-xiang, Yang Guang-de, Liu Yu, Zhang Hui
Department of Pharmacology, Xi'an Jiaotong University, School of Medicine, Xi'an, Shaanxi, PR China.
J Ethnopharmacol. 2008 Jan 17;115(2):217-22. doi: 10.1016/j.jep.2007.09.025. Epub 2007 Oct 3.
Tanshinone IIA (Tan IIA) is a member of the major lipophilic components abstracted from the root of Salvia miltiorrhiza Bunge and has the capacity of anti-atherosclerosis. To investigate the potential mechanism, we established an animal model by giving high fatty diet to rabbits and Tan IIA was given in different dose. Then, superoxide dismutase (SOD) activity and the malondialdehyde (MDA) level in serum were detected using spectrophotometry; cluster of differentiation 40 (CD40) expression of cellular membrane fraction of aortas and matrix metalloproteinase-2 (MMP-2) activity of total protein extract of aortas were detected by Western Blotting and Zymography, respectively. Compared with the control group, the level of MDA, the expression of CD40 and the MMP-2 activity were increased while the SOD activity was decreased significantly in model group. After Tan IIA administration, the SOD activity was significantly increased while the level of MDA was decreased; both the expression of CD40 and the activity of MMP-2 were decreased. It is suggested that Tan IIA not only inhibits the oxidation but also suppresses the inflammation in atherosclerotic lesion. Our data suggest that not only anti-oxidation but also anti-inflammation by decrease the expression of CD40 and MMP-2 activity maybe the potential mechanisms by which Tan IIA anti-atherosclerosis.
丹参酮IIA（Tan IIA）是从丹参根中提取的主要亲脂性成分之一，具有抗动脉粥样硬化的能力。为了探究其潜在机制，我们通过给兔子喂食高脂饮食建立了动物模型，并给予不同剂量的Tan IIA。然后，采用分光光度法检测血清中超氧化物歧化酶（SOD）活性和丙二醛（MDA）水平；分别通过蛋白质免疫印迹法和酶谱法检测主动脉细胞膜部分的分化簇40（CD40）表达以及主动脉总蛋白提取物的基质金属蛋白酶-2（MMP-2）活性。与对照组相比，模型组中MDA水平、CD40表达和MMP-2活性升高，而SOD活性显著降低。给予Tan IIA后，SOD活性显著升高，MDA水平降低；CD40表达和MMP-2活性均降低。提示Tan IIA不仅抑制氧化，还抑制动脉粥样硬化病变中的炎症。我们的数据表明，不仅抗氧化，而且通过降低CD40表达和MMP-2活性来抗炎可能是Tan IIA抗动脉粥样硬化的潜在机制。
