印度加尔各答分离出的河流弧菌克隆相关临床分离株中的耐药机制
Mechanism of drug resistance in clonally related clinical isolates of Vibrio fluvialis isolated in Kolkata, India.
作者信息
Srinivasan Vijaya Bharathi, Virk Rupinder Kaur, Kaundal Amita, Chakraborty Rupa, Datta Basabjit, Ramamurthy T, Mukhopadhyay Asish K, Ghosh Amit
机构信息
Institute of Microbial Technology, Molecular Biology Division, Sector 39A, Chandigarh 160036, India.
出版信息
Antimicrob Agents Chemother. 2006 Jul;50(7):2428-32. doi: 10.1128/AAC.01561-05.
Abstract
The molecular mechanisms of drug resistance in 19 strains of Vibrio fluvialis isolated from 1998 to 2002 in Kolkata, India, were investigated. Class 1 integrons were detected in eight strains, and four strains were found to carry SXT integrases. In the presence of carbonyl cyanide m-chlorophenylhydrazone or reserpine, all nalidixic acid- and ciprofloxacin-resistant strains became sensitive, suggesting that drug efflux plays a major role in quinolone resistance in V. fluvialis. It was further seen that strains which had MICs of > 25 microg/ml for nalidixic acid had a sense mutation (Ser to Ile) at position 83 of the quinolone resistance-determining region of gyrA. All except one of the integron- and SXT integrase-bearing strains belonged to the same ribotype.
摘要
对1998年至2002年从印度加尔各答分离出的19株河流弧菌的耐药分子机制进行了研究。在8株菌株中检测到1类整合子，发现4株携带SXT整合酶。在存在羰基氰化物间氯苯腙或利血平的情况下，所有对萘啶酸和环丙沙星耐药的菌株都变得敏感，这表明药物外排在河流弧菌对喹诺酮类药物的耐药性中起主要作用。进一步观察到，对萘啶酸的最低抑菌浓度>25μg/ml的菌株在gyrA喹诺酮耐药决定区的83位发生了一个有义突变（丝氨酸突变为异亮氨酸）。除一株外，所有携带整合子和SXT整合酶的菌株都属于同一核糖体分型。
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FEMS Microbiol Lett. 2005 Jan 15;242(2):241-7. doi: 10.1016/j.femsle.2004.11.012.
2
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J Antimicrob Chemother. 2004 Jun;53(6):947-51. doi: 10.1093/jac/dkh221. Epub 2004 Apr 29.
3
Class I integrons and SXT elements in El Tor strains isolated before and after 1992 Vibrio cholerae O139 outbreak, Calcutta, India.1992年印度加尔各答霍乱弧菌O139暴发前后分离的埃尔托菌株中的I类整合子和SXT元件
Emerg Infect Dis. 2003 Apr;9(4):500-2. doi: 10.3201/eid0904.020317.
4
Occurrence of antibiotic resistance gene cassettes aac(6')-Ib, dfrA5, dfrA12, and ereA2 in class I integrons in non-O1, non-O139 Vibrio cholerae strains in India.印度非O1、非O139霍乱弧菌菌株中I类整合子内抗生素抗性基因盒aac(6')-Ib、dfrA5、dfrA12和ereA2的出现情况
Antimicrob Agents Chemother. 2002 Sep;46(9):2948-55. doi: 10.1128/AAC.46.9.2948-2955.2002.
5
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