Wang Xiu-Juan, Bai Ji-Gang, Liang Yun-Xiang
The Key Laboratory of Crop Biology of Shandong, College of Life Science, Shandong Agricultural University, Tai'an 271018, People's Republic of China.
Appl Microbiol Biotechnol. 2006 Dec;73(3):533-40. doi: 10.1007/s00253-006-0496-1. Epub 2006 Jun 27.
F(3) and F(4) strains of Aspergillus niger were screened from five strains of fungi to produce multienzyme preparations (containing cellulase, hemicellulase, glucoamylase, pectinase, and acidic proteinase) as dietary supplementation. Enzyme activities indicated that 1:4 (F(3) to F(4)) was the optimum mixture proportion, and 0.3% (W/W) was the preferable pitching rate. In bran mash containing 54.5% (W/W) water, F(3) and F(4) could produce the supplementation better when cultured 30 to 36 h at 30 degrees C. Monofactorial and orthogonal experiments were performed to optimize media. Results of the variance and range analysis showed that the optimum medium contained 80 g of bran, 20 g of cottonseed powder, 1 g of (NH(4))(2)SO(4), and 0.1 g of KH(2)PO(4). When F(3) and F(4 )strains were cultured in the optimum medium containing 54.5% (W/W) water, the activity of cellulase, hemicellulase, glucoamylase, pectinase, and acidic proteinase reached 996; 15,863; 13,378; 7,621; and 5,583 U/g, respectively.
从五株真菌中筛选出黑曲霉F(3)和F(4)菌株，用于生产多酶制剂（包含纤维素酶、半纤维素酶、糖化酶、果胶酶和酸性蛋白酶）作为饲料添加剂。酶活性表明1:4（F(3)与F(4)）为最佳混合比例，0.3%（W/W）为最佳接种量。在含水量为54.5%（W/W）的麸皮醪中，F(3)和F(4)在30℃培养30至36小时时能更好地生产添加剂。进行单因素和正交试验以优化培养基。方差分析和极差分析结果表明，最佳培养基包含80克麸皮、20克棉籽粉、1克硫酸铵和0.1克磷酸二氢钾。当F(3)和F(4)菌株在含水量为54.5%（W/W）的最佳培养基中培养时，纤维素酶、半纤维素酶、糖化酶、果胶酶和酸性蛋白酶的活性分别达到996、15863、13378、7621和5583 U/g。
