Oghobase G E, Aladesanmi O T, Akomolafe R O, Olukiran O S, Akano P O, Eimunjeze M H
Institute of Ecology and Environmental Studies, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
Department of Physiological Sciences, Faculty of Basic Medical Sciences, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
Toxicol Rep. 2020 Aug 22;7:1103-1111. doi: 10.1016/j.toxrep.2020.08.007. eCollection 2020.
Fufu is a major component of food in West Africa. Local cassava farmers use detergent to ferment cassava root tuber during processing into fufu. Studies have established the hazardous health effects of detergent in humans. This study was carried out to determine the median lethal toxicity of detergent-processed cassava, and assessed its effects on the renal function of male Wistar rats. Fufu with different concentrations of detergent (250, 500 and 750 g/l) were given as food twice daily to the rats for the acute toxicity study. The sub-chronic test lasted for four weeks, during which the fufu samples prepared with 10, 20, 30, 40 and 50 g/l of detergent, were separately given to five groups of five rats, respectively. The results of the acute toxicity test showed increase in mortality with corresponding increase in detergent concentration. Plasma and urine creatinine and urea concentrations of all the detergent-treated rats were significantly higher than that of the negative and positive controls. Urinalysis showed significant presence of glucose and ketone bodies, as well as, blood and protein. Renal photomicrographs showed deranged cortical structure with diffuse loss of tubules, inflammatory cells infiltrate in the interstitium. The study concluded that detergent-processed cassava has adverse effects on the structure and function of the kidneys of rats.
富富是西非食物的主要组成部分。当地木薯种植户在将木薯块根加工成富富的过程中使用洗涤剂发酵。研究已证实洗涤剂对人体有有害健康影响。本研究旨在确定用洗涤剂处理的木薯的半数致死毒性，并评估其对雄性Wistar大鼠肾功能的影响。在急性毒性研究中，将含有不同浓度洗涤剂（250、500和750克/升）的富富作为食物，每天分两次喂给大鼠。亚慢性试验持续四周，在此期间，将分别用10、20、30、40和50克/升洗涤剂制备的富富样品分别喂给五组，每组五只大鼠。急性毒性试验结果显示，死亡率随洗涤剂浓度的增加而上升。所有经洗涤剂处理的大鼠的血浆和尿液肌酐及尿素浓度均显著高于阴性和阳性对照组。尿液分析显示葡萄糖、酮体、血液和蛋白质大量存在。肾脏显微照片显示皮质结构紊乱，肾小管弥漫性丧失，间质中有炎性细胞浸润。该研究得出结论，用洗涤剂处理的木薯对大鼠肾脏的结构和功能有不良影响。
