Nie Rui, Sunmonu N Abimbola, Chin Anne B, Lee Kyungmouk S, Rutkove Seward B
Department of Neurology, Division of Neuromuscular Diseases, Harvard Medical School, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, TCC 810, Boston, MA 02215, USA.
Clin Neurophysiol. 2006 Aug;117(8):1844-9. doi: 10.1016/j.clinph.2006.03.024. Epub 2006 Jun 30.
To determine the feasibility of performing electrical impedance myography (EIM) in rats.
EIM was performed on the hamstring muscles of 6 healthy adult rats with applied frequencies of 2-300 kHz. Studies were performed over a 6-week period, with 3 rats having recordings made from the skin (surface EIM) and 3 with recordings directly from the muscle (direct-muscle EIM). In addition, sciatic nerve crush was performed on one rat and comparisons made pre- and post-injury. Reactance and resistance were measured and the primary outcome variable, the phase angle (theta), calculated.
EIM patterns in the rat hamstring muscles were qualitatively similar to those observed in human subjects. This held true for both surface and direct-muscle recordings, although direct-muscle data appeared less repeatable. Sciatic nerve crush data in the single rat showed a dramatic reduction in phase and a relative loss of frequency-dependence.
EIM data similar to that obtained from human subjects can be acquired from rat muscles with surface recordings proving more consistent and easier to obtain than direct-muscle recordings. Changes seen with sciatic nerve crush mirror those seen in patients with neurogenic injury.
These results support the possibility of performing EIM on rat models of neuromuscular disease.
确定在大鼠中进行电阻抗肌电图（EIM）检查的可行性。
对6只健康成年大鼠的腘绳肌进行EIM检查，施加频率为2 - 300 kHz。研究为期6周，3只大鼠从皮肤进行记录（表面EIM），3只直接从肌肉进行记录（直接肌肉EIM）。此外，对1只大鼠进行坐骨神经挤压，并在损伤前后进行比较。测量电抗和电阻，并计算主要结果变量相角（θ）。
大鼠腘绳肌的EIM模式在质量上与在人类受试者中观察到的相似。表面记录和直接肌肉记录均如此，尽管直接肌肉数据的重复性似乎较低。单只大鼠的坐骨神经挤压数据显示相角显著降低以及频率依赖性相对丧失。
可以从大鼠肌肉中获取与人类受试者相似的EIM数据，表面记录比直接肌肉记录更一致且更容易获得。坐骨神经挤压所见变化与神经源性损伤患者所见相似。
这些结果支持在神经肌肉疾病大鼠模型上进行EIM检查的可能性。
