Ahad Mohammad A, Rutkove Seward B
Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Clin Neurophysiol. 2009 Aug;120(8):1534-8. doi: 10.1016/j.clinph.2009.05.017. Epub 2009 Jun 30.
To describe a refined technique for performing electrical impedance myography (EIM) in the rat and assess its reproducibility, long-term stability, and the effects of sciatic nerve injury.
EIM at 50kHz was performed on the gastrocnemius-soleus complex of the rat hind limb in 12 rats, followed from 6 weeks of age for up to 6 months. Eight additional rats underwent sciatic nerve crush and 6 underwent a sham procedure.
The EIM variables of resistance, reactance and phase demonstrated substantial change with growth until approximately 14 weeks of age, at which point the measurements stabilized, giving mean values of 6.0+/-5.7Omega, 22.1+/-2.1Omega, and 16.5+/-1.1 degrees , respectively, at 16 weeks of age. Immediate reproducibility of technique was high with an intraclass correlation coefficient of 0.91 and higher for all three parameters. Sciatic crush produced marked reductions in the reactance and phase that reversed over a several week period.
These results support that 50kHz EIM can be performed effectively in adult rat models of neuromuscular disease with a straightforward experimental technique and that it is sensitive to neurogenic injury.
EIM can serve as a new approach to the study of neuromuscular disease in the rat.
描述一种在大鼠中进行电阻抗肌电图（EIM）的精细技术，并评估其可重复性、长期稳定性以及坐骨神经损伤的影响。
对12只大鼠后肢的腓肠肌 - 比目鱼肌复合体进行50kHz的EIM检测，从6周龄开始持续6个月。另外8只大鼠接受坐骨神经挤压，6只大鼠接受假手术。
电阻、电抗和相位的EIM变量在约14周龄前随生长有显著变化，之后测量值稳定，在16周龄时平均值分别为6.0±5.7Ω、22.1±2.1Ω和16.5±1.1度。该技术的即时可重复性很高，所有三个参数的组内相关系数均为0.91及以上。坐骨神经挤压导致电抗和相位显著降低，在几周内恢复。
这些结果支持50kHz EIM可以通过简单的实验技术在成年大鼠神经肌肉疾病模型中有效进行，并且它对神经源性损伤敏感。
EIM可作为研究大鼠神经肌肉疾病的一种新方法。
