Magnusardottir Anna R, Skuladottir Gudrun V
Department of Physiology, Faculty of Medicine, University of Iceland, Reykjavik.
Lipids. 2006 Apr;41(4):401-4. doi: 10.1007/s11745-006-5112-8.
The stability of PUFA in venous red blood cells (RBC) of women aged 25 to 55 years (n = 12) was investigated during storage at -20 degrees C. The RBC sample from each participant was divided into seven portions: one baseline with the antioxidant BHT, another without BHT, samples without BHT stored for 2, 4, 9, or 17 wk, and samples with BHT stored for 17 wk. No difference was found in proportions of PUFA at baseline and after storage for 2 and 4 wk without BHT, and 17 wk with BHT. After 9 wk without BHT the proportion of 22:6n-3 in RBC was lower, and after 17 wk without BHT proportions of all PUFA were lower than at baseline. High proportion of 22:6n-3 in RBC at baseline was associated with more stable concentration of total FA in RBC without BHT during 17 wk. The findings indicate that PUFA in RBC from healthy women are stable at -20 degrees C for 4 wk without BHT and for at least 17 wk with BHT.
研究了25至55岁女性（n = 12）静脉红细胞（RBC）中多不饱和脂肪酸（PUFA）在-20℃储存期间的稳定性。将每位参与者的RBC样本分成七份：一份添加抗氧化剂丁基羟基甲苯（BHT）作为基线样本，另一份不添加BHT，不添加BHT的样本分别储存2、4、9或17周，添加BHT的样本储存17周。在基线时以及不添加BHT储存2周和4周、添加BHT储存17周后，PUFA的比例没有差异。不添加BHT储存9周后，RBC中22:6n-3的比例降低，不添加BHT储存17周后，所有PUFA的比例均低于基线水平。基线时RBC中22:6n-3的高比例与不添加BHT时RBC中总脂肪酸在17周内更稳定的浓度相关。研究结果表明，健康女性RBC中的PUFA在-20℃下，不添加BHT时可稳定4周，添加BHT时可稳定至少17周。
