Hansen Christopher J, Burnell Kindra K, Brogden Kim A
Department of Periodontics and Dows Institute for Dental Research, The University of Iowa College of Dentistry, Iowa City, IA 52252, USA.
J Neuroimmunol. 2006 Aug;177(1-2):215-8. doi: 10.1016/j.jneuroim.2006.05.011. Epub 2006 Jun 30.
Infection and inflammation of mucosal tissue may induce the production of neuropeptides, specifically Substance P and Neuropeptide Y. Since these neuropeptides are similar to antimicrobial peptides in their amino acid composition, amphipathic design, cationic charge, and size, we wanted to determine if they had antimicrobial activity against a panel of common bacteria and oral microorganisms using the radial diffusion assay. Neuropeptide Y and Substance P had antimicrobial activity against E. coli (MIC 20.6+/-5.5 microg/ml SEM and 71.5+/-15 SEM microg/ml, respectively), but did not have activity against laboratory strains of Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Serratia marcescens (MIC>500 microg/ml) nor oral strains of Streptococcus mutans, Candida albicans, and Actinobacillus actinomycetemcomitans (MIC>500 microg/ml). While Substance P and Neuropeptide Y did not have direct antimicrobial activity against the microorganisms tested, they still may stimulate local epithelial cells to produce other innate immune factors like defensins and cathelicidins. However, this remains to be determined.
黏膜组织的感染和炎症可能会诱导神经肽的产生，特别是P物质和神经肽Y。由于这些神经肽在氨基酸组成、两亲性设计、阳离子电荷和大小方面与抗菌肽相似，我们想用径向扩散试验来确定它们对一组常见细菌和口腔微生物是否具有抗菌活性。神经肽Y和P物质对大肠杆菌具有抗菌活性（MIC分别为20.6±5.5微克/毫升标准误和71.5±15标准误微克/毫升），但对金黄色葡萄球菌、铜绿假单胞菌、肺炎克雷伯菌和粘质沙雷氏菌的实验室菌株（MIC>500微克/毫升）以及变形链球菌、白色念珠菌和伴放线放线杆菌的口腔菌株（MIC>500微克/毫升）没有活性。虽然P物质和神经肽Y对所测试的微生物没有直接的抗菌活性，但它们仍可能刺激局部上皮细胞产生其他固有免疫因子，如防御素和cathelicidins。然而，这仍有待确定。
