Santoli D, Wroblewska Z, Gilden D H, Girardi A, Koprowski H
J Comp Neurol. 1975 Jun 1;161(3):317-28. doi: 10.1002/cne.901610304.
Cells from explants and monolayer subcultures of adult human brain obtained from biopsies or autopsies of ten multiple sclerosis (MS) cases, one case each of Jakob-Creutzfeld (JC) and amyotrophic lateral sclerosis (ALS) and three cases with no central nervous system (CNS) involvement were transformed with PML-SV40 virus. Transformation was effected to establish permanent lines of these particular adult brain cells so that sufficient quantities would be available for other research projects. The procedure previously used to transform human fibroblasts (Koprowski et al., '62) was successfully applied to human brain cells. The success of transformation was dependent on the growth condition of the cultures at the time of infection. Events occurring after viral infection and during the pre-transformation and the post-transformation phases are described.
从10例多发性硬化症（MS）患者的活检或尸检、1例亚急性硬化性全脑炎（JC）和1例肌萎缩侧索硬化症（ALS）患者以及3例无中枢神经系统（CNS）受累患者获取的成人人类脑组织外植体和单层传代培养细胞，用PML - SV40病毒进行转化。进行转化是为了建立这些特定成人脑细胞的永久细胞系，以便为其他研究项目提供足够数量的细胞。先前用于转化人成纤维细胞的方法（Koprowski等人，1962年）成功应用于人类脑细胞。转化的成功取决于感染时培养物的生长条件。描述了病毒感染后以及转化前和转化后阶段发生的事件。
