埃泽穆尔盐红菌新种，一种从阿尔及利亚埃泽穆尔盐沼分离出的嗜盐古菌。
Halorubrum ezzemoulense sp. nov., a halophilic archaeon isolated from Ezzemoul sabkha, Algeria.
作者信息
Kharroub Karima, Quesada Teresa, Ferrer Raquel, Fuentes Susana, Aguilera Margarita, Boulahrouf Abdrahmane, Ramos-Cormenzana Alberto, Monteoliva-Sánchez Mercedes
机构信息
Institut de Nutrition de l'Alimentation et des Technologies Agro-Alimentaires, Université Mentouri, Constantine, Algeria.
Departamento de Microbiologia, Facultad de Farmacia, Campus Universitario de Cartuja s/n 18071, Granada, Spain.
出版信息
Int J Syst Evol Microbiol. 2006 Jul;56(Pt 7):1583-1588. doi: 10.1099/ijs.0.64272-0.
A novel extremely halophilic archaeon was isolated from Ezzemoul sabkha, Algeria. The strain, designated 5.1(T), was neutrophilic, motile and Gram-negative. At least 15 % (w/v) NaCl was required for growth. The isolate grew at pH 6.5-9.0, with optimum growth at pH 7.0-7.5. Mg(2+) was required for growth. Polar lipids were C(20)C(20) derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, and phosphatidylglycerol sulfate and sulfated diglycosyl diether. The genomic DNA G+C content of strain 5.1(T) was 61.9 mol% (T(m)). Phylogenetic analysis based on comparison of 16S rRNA gene sequences revealed that strain 5.1(T) clustered with Halorubrum species. The results of DNA-DNA hybridization and biochemical tests allowed genotypic and phenotypic differentiation of strain 5.1(T) from other Halorubrum species. The name Halorubrum ezzemoulense sp. nov. (type strain 5.1(T)=CECT 7099(T)=DSM 17463(T)) is proposed.
从阿尔及利亚的埃泽穆尔盐沼分离出一株新型极端嗜盐古菌。该菌株命名为5.1(T)，为嗜中性、可运动且革兰氏阴性。生长需要至少15%（w/v）的NaCl。该分离株在pH 6.5 - 9.0范围内生长，最适生长pH为7.0 - 7.5。生长需要Mg(2+)。极性脂质为磷脂酰甘油、磷脂酰甘油磷酸甲酯、磷脂酰甘油硫酸酯和硫酸化二糖基二醚的C(20)C(20)衍生物。菌株5.1(T)的基因组DNA G + C含量为61.9 mol%（T(m)）。基于16S rRNA基因序列比较的系统发育分析表明，菌株5.1(T)与嗜盐红菌属物种聚类在一起。DNA - DNA杂交和生化试验结果使菌株5.1(T)能够与其他嗜盐红菌属物种进行基因型和表型区分。提出了新种埃泽穆尔嗜盐红菌（Halorubrum ezzemoulense sp. nov.）（模式菌株5.1(T)=CECT 7099(T)=DSM 17463(T)）。
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