Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea.
Division of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea.
J Microbiol. 2022 Oct;60(10):1007-1020. doi: 10.1007/s12275-022-2173-1. Epub 2022 Aug 26.
A novel haloarchaeal species designated as MBLA0099 was isolated from seawater near Yeongheung Island. Cells were Gram-negative, non-motile, red-pigmented, and rod-shaped. They grew at 10-45°C, within pH 5.5-9.0, and between 7.5% and 30% NaCl concentrations. Cells were able to grow without Mg and were lysed in distilled water. The size of the whole-genome and G + C content of DNA was 3.02 Mb and 68.9 mol%, respectively. Phylogenetic analysis shows that the strain MBLA0099 belongs to the genus Halorubrum. The average nucleotide and amino acid identity, and in silico DNA-DNA hybridization values were below the species delineation threshold. Pan-genomic analysis revealed that 3.2% of all genes present in strain MBLA0099 were unique to the strain. The red carotenoid produced by strain MBLA0099 was subjected to spectrometric and chromatographic analyses and confirmed to be bacterioruberin as C50 carotenoid. Mevalonic acid, terpenoid backbone, and carotenoid biosynthesis pathway were annotated for strain MBLA0099. The C50 carotenoid production by strain MBLA0099 was also enhanced under various stress conditions including relatively netural pH, high oxidative and salinity conditions. Additionally, the strain MBLA0099-derived bacterioruberin showed the antioxidant activity with EC value of 12.29 µg/ml, based on the evaluation of DPPH free radical scavenging activity. The present study would be the first report on the identification of C carotenoid from the strain MBLA0099 representing a novel species of the genus Halorubrum, for which the name Halorubrum ruber sp. nov. is proposed. The typestrain used was MBLA0099 (= KCTC 4296 = JCM 34701).
一种新型的盐杆菌属物种，命名为 MBLA0099，是从永宗岛附近的海水中分离得到的。细胞呈革兰氏阴性、非运动性、红色、杆状。它们在 10-45°C、pH5.5-9.0、7.5%-30%NaCl 浓度范围内生长。细胞能够在没有镁的情况下生长，并在蒸馏水中溶解。全基因组大小和 DNA G+C 含量分别为 3.02 Mb 和 68.9 mol%。系统发育分析表明，该菌株 MBLA0099 属于盐杆菌属。平均核苷酸和氨基酸同一性以及 DNA-DNA 杂交值低于种的划定阈值。泛基因组分析显示，MBLA0099 菌株中 3.2%的基因是该菌株所特有的。MBLA0099 菌株产生的红色类胡萝卜素经过光谱和色谱分析，确认为 C50 类胡萝卜素菌红菌素。MBLA0099 菌株的甲羟戊酸、萜烯骨架和类胡萝卜素生物合成途径得到了注释。在各种胁迫条件下，包括相对中性 pH 值、高氧化和高盐条件下，MBLA0099 菌株的 C50 类胡萝卜素产量也得到了提高。此外，MBLA0099 衍生的菌红菌素表现出抗氧化活性，根据 DPPH 自由基清除活性的评估，其 EC 值为 12.29 µg/ml。本研究首次报道了从代表盐杆菌属新种的 MBLA0099 菌株中鉴定出 C 类胡萝卜素，该新种命名为 Halorubrum ruber sp. nov.。使用的模式菌株为 MBLA0099（=KCTC 4296=JCM 34701）。
