Yamada Yumiko, Yasui Hiroyuki, Sakurai Hiromu
Department of Analytical and Bioinorganic Chemistry, Kyoto Pharmaceutical University, Kyoto, Japan.
Photochem Photobiol. 2006 Nov-Dec;82(6):1668-76. doi: 10.1562/2006-03-24-RA-857.
Caffeic acid (CA) and its analogues such as rosmarinic acid are well known as antioxidative agents. Exposure to UVA is known to generate reactive oxygen species (ROS) such as singlet oxygen (1O2) and superoxide anion radical (*O2-) in the skin of animals, which in turn induces skin photodamage and photoaging. Because CA and its analogues quench 1O2, these compounds were topically applied to the abdominal skin of live hairless mice and were found to suppress ROS generation upon UVA exposure. Furthermore, the generation of UVA-induced ROS was also suppressed in the skin of mice that were orally given CA. In order to understand the mechanism by which CA blocks ROS production in UVA-exposed skin, the pharmacokinetics of CA upon oral administration to mice was followed and CA was found to efficiently distribute in the skin. These results suggest that skin damage by UVA-induced ROS generation is reduced by oral supplementation of CA, which has a scavenging and quenching activity against ROS.
咖啡酸（CA）及其类似物如迷迭香酸作为抗氧化剂广为人知。已知暴露于紫外线A（UVA）会在动物皮肤中产生活性氧（ROS），如单线态氧（1O2）和超氧阴离子自由基（*O2-），进而导致皮肤光损伤和光老化。由于CA及其类似物能淬灭1O2，因此将这些化合物局部涂抹于活体无毛小鼠的腹部皮肤，发现其能抑制UVA照射后ROS的产生。此外，口服CA的小鼠皮肤中UVA诱导的ROS生成也受到抑制。为了了解CA阻断UVA照射皮肤中ROS产生的机制，对口服给予小鼠CA后的药代动力学进行了跟踪，发现CA能有效分布于皮肤中。这些结果表明，口服补充具有清除和淬灭ROS活性的CA可减少UVA诱导的ROS产生对皮肤的损伤。
