Ribeiro J, Almeida-Dias A, Ascensão A, Magalhães J, Oliveira A R, Carlson J, Mota J, Appell H-J, Duarte J
EsEF/UFRGS Physical Education School, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
J Sci Med Sport. 2007 Jun;10(3):164-9. doi: 10.1016/j.jsams.2006.06.001. Epub 2006 Jul 17.
The chronic and immediate post-exercise responses in the hemostatic and fibrinolytic systems have been shown to be variable and reflect differing adaptations with ageing and responses to exercise protocols. This study investigated the effects of acute and exhaustive exercise on the amplitude and duration of hemostatic and fibrinolytic responses in young adolescent males. The sample comprised 10 sedentary boys (13.2+/-0.5 years, 55.8+/-11.3kg, 165.7+/-7.4cm), who had not exercised or received any medication for at least 2 weeks before the experiments. The subjects performed exhaustive stepping exercise, consisting of 1s up and down cycles to fatigue. When the subjects were unable to maintain the required stepping rhythm, they were given a 30s recovery period. Following each 30s recovery participants recommenced the stepping cadence until fatigue prevented them continuing. Venous blood samples were drawn before and immediately, 1 and 24h after exercise to assess the following coagulation and fibrinolytic parameters: Platelet counts, activated partial thromboplastin time (aPTT), prothrombin time (PT), coagulation factor VIII (FVIII:C), von Willebrand factor (vWF), fibrinogen concentration, thrombin-antithrombin complex (TAT), D-dimer, plasminogen activator inhibitor (PAI-1), and tissue-type plasminogen activator (t-PA). Immediately following exercise, platelet counts, aPTT, FVIII, vWF and t-PA were significantly elevated in contrast to PAI-1, which decreased significantly until 1h after exercise. FVIII and platelet counts were elevated at 1 and 24h after exercise, respectively. Only the parameters FVIII and PAI-1 did not return to baseline values during the first hour after physical exercise. When compared to adults the results revealed different rates and ranges of coagulation and fibrinolysis parameters being activated by exhaustive exercise in this group of adolescents.
止血和纤溶系统的慢性及运动后即时反应已被证明具有变异性，反映出随着年龄增长的不同适应性以及对运动方案的反应。本研究调查了急性力竭运动对年轻青少年男性止血和纤溶反应的幅度及持续时间的影响。样本包括10名久坐不动的男孩（13.2±0.5岁，55.8±11.3kg，165.7±7.4cm），他们在实验前至少2周未进行运动或服用任何药物。受试者进行力竭性踏步运动，由每秒上下循环直至疲劳。当受试者无法维持所需的踏步节奏时，给予30秒的恢复期。每30秒恢复后，参与者重新开始踏步节奏，直至疲劳使其无法继续。在运动前、运动后即刻、1小时和24小时采集静脉血样本，以评估以下凝血和纤溶参数：血小板计数、活化部分凝血活酶时间（aPTT）、凝血酶原时间（PT）、凝血因子VIII（FVIII:C）、血管性血友病因子（vWF）、纤维蛋白原浓度、凝血酶 - 抗凝血酶复合物（TAT）、D - 二聚体、纤溶酶原激活物抑制剂（PAI - 1）和组织型纤溶酶原激活物（t - PA）。运动后即刻，与PAI - 1相比，血小板计数、aPTT、FVIII、vWF和t - PA显著升高，PAI - 1在运动后1小时前显著降低。运动后1小时和24小时，FVIII和血小板计数分别升高。在体育锻炼后的第一小时内，只有FVIII和PAI - 1参数未恢复到基线值。与成年人相比，结果显示在这组青少年中，力竭运动激活凝血和纤溶参数的速率和范围不同。
