在HIV-1感染个体的脑和淋巴结区室中不同病毒基因型占主导地位。
Predominance of distinct viral genotypes in brain and lymph node compartments of HIV-1-infected individuals.
作者信息
Haggerty S, Stevenson M
机构信息
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha.
出版信息
Viral Immunol. 1991 Summer;4(2):123-31. doi: 10.1089/vim.1991.4.123.
Abstract
A modified polymerase chain reaction protocol was used to amplify the entire envelope-coding region of HIV-1 directly from brain and lymph node tissue obtained at autopsy from three HIV-1-infected individuals. Molecular analysis of amplified DNA by digestion with 18 restriction endonucleases, singly and in combination, revealed different HIV-1 genotypes in the brain and lymph node compartments in each of the three individuals. This anatomic compartmentalization of HIV-1 populations may reflect different viral genomic sequences that determine tropism or differences in host immune selection pressures in the brain and lymphoid compartments that drive the emergence of distinct viral populations.
摘要
采用改良的聚合酶链反应方案，直接从三名感染HIV-1个体尸检时获得的脑和淋巴结组织中扩增HIV-1的整个包膜编码区。通过用18种限制性内切酶单独或联合消化对扩增的DNA进行分子分析，发现这三名个体的脑和淋巴结区室中存在不同的HIV-1基因型。HIV-1群体的这种解剖学分区可能反映了决定嗜性的不同病毒基因组序列，或者反映了脑和淋巴区室中宿主免疫选择压力的差异，这些差异推动了不同病毒群体的出现。
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