Topal Ummihan, Sasaki Mitsuru, Goto Motonobu, Hayakawa Kiro
Department of Applied Chemistry and Biochemistry, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan.
J Agric Food Chem. 2006 Jul 26;54(15):5604-10. doi: 10.1021/jf0606407.
Supercritical carbon dioxide (SCCO2) extraction of lycopene from waste tomato skins was investigated. The experiments were carried out at pressures and temperatures ranging from 20 to 50 MPa and 313 to 373 K, respectively, without any modifiers. The flow rate of CO2 was maintained at 2.5 mL/min for 330 min extraction time. Solvent flow rate effect was examined for CO2 flow rates from 1.5 to 4.5 mL/min. The extracts were analyzed by high-performance liquid chromatography and UV-visible spectroscopy. The results showed that with optimized operating conditions, the maximum yield of lycopene (1.18 mg of lycopene/g of sample) was obtained at 40 MPa, 373 K, and 2.5 mL of CO2/min. Chromatographic analysis indicated that lycopene was extracted from tomato skin with negligible degradation at the optimum conditions and the amount extracted represented more than 94% of the total carotenoid content of the sample. The solubility of lycopene was modeled by use of the Chrastil equation.
研究了用超临界二氧化碳（SCCO2）从废弃番茄皮中提取番茄红素。实验分别在压力为20至50MPa、温度为313至373K的条件下进行，未使用任何改性剂。二氧化碳流速在330分钟的萃取时间内保持为2.5mL/分钟。考察了1.5至4.5mL/分钟的二氧化碳流速对溶剂流速的影响。提取物通过高效液相色谱法和紫外可见光谱法进行分析。结果表明，在优化的操作条件下，在40MPa、373K和2.5mL二氧化碳/分钟的条件下获得了番茄红素的最大产量（1.18mg番茄红素/克样品）。色谱分析表明，在最佳条件下从番茄皮中提取的番茄红素降解可忽略不计，提取量占样品总类胡萝卜素含量的94%以上。利用Chrastil方程对番茄红素的溶解度进行了建模。
