Acín Sergio, Navarro María A, Arbonés-Mainar José M, Guillén Natalia, Sarría Alfonso J, Carnicer Ricardo, Surra Joaquín C, Orman Israel, Segovia Jose C, Torre Rafael de la, Covas María-Isabel, Fernández-Bolaños Juan, Ruiz-Gutiérrez Valentina, Osada Jesús
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Universidad de Zaragoza.
J Biochem. 2006 Sep;140(3):383-91. doi: 10.1093/jb/mvj166. Epub 2006 Jul 27.
Hydroxytyrosol is a phenol found in olive oil. To verify the effect of hydroxytyrosol on the development of atherosclerosis, two groups of apo E deficient male mice on a standard chow diet were used: the control group receiving only water, and the second group an aqueous solution of hydroxytyrosol in order to provide a dose of 10 mg/kg/day to each mouse. This treatment was maintained for 10 weeks. At the moment of sacrifice, blood was drawn and heart removed. Plasma lipids, apolipoproteins and monocyte Mac-1 expression were assayed as well as aortic atherosclerotic areas in both groups. Data showed no significant changes in HDL cholesterol, paraoxonase, apolipoprotein B or triglyceride levels. However, hydroxytyrosol administration decreased apolipoprotein A-I and increased total cholesterol, atherosclerotic lesion areas and circulating monocytes expressing Mac-1. The latter was highly correlated with lesion areas (r = 0.65, P < 0.01). These results indicate that administration of hydroxytyrosol in low cholesterol diets increases atherosclerotic lesion associated with the degree of monocyte activation and remodelling of plasma lipoproteins. Our data supports the concept that phenolic-enriched products, out of the original matrix, could be not only non useful but also harmful. Our results suggest that the formulation of possible functional foods should approximate as much as possible the natural environment in which active molecules are found.
羟基酪醇是一种存在于橄榄油中的酚类物质。为了验证羟基酪醇对动脉粥样硬化发展的影响，研究人员使用了两组以标准饲料喂养的载脂蛋白E缺陷雄性小鼠：对照组仅给予水，第二组给予羟基酪醇水溶液，以便每只小鼠每天摄入10毫克/千克的剂量。这种治疗持续了10周。在处死小鼠时，采集血液并取出心脏。对两组小鼠的血浆脂质、载脂蛋白和单核细胞Mac-1表达进行了检测，同时也检测了主动脉粥样硬化区域。数据显示，高密度脂蛋白胆固醇、对氧磷酶、载脂蛋白B或甘油三酯水平没有显著变化。然而，给予羟基酪醇会降低载脂蛋白A-I，并增加总胆固醇、动脉粥样硬化病变区域以及表达Mac-1的循环单核细胞。后者与病变区域高度相关（r = 0.65，P < 0.01）。这些结果表明，在低胆固醇饮食中给予羟基酪醇会增加与单核细胞活化程度和血浆脂蛋白重塑相关的动脉粥样硬化病变。我们的数据支持这样一种观点，即脱离原始基质的富含酚类的产品不仅可能无用，而且可能有害。我们的结果表明，可能的功能性食品的配方应尽可能接近发现活性分子的自然环境。
